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Example sentences for: tem
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Thirty-six percent represents a minimum estimate of TEM1 bypass efficiency in tab3-1 cultures, since cells with 1-2 cell bodies could have arisen from bypass events followed by successful cell separation.
Cdc5 is required for full activation of the Dbf2-Mob1 protein kinase complex [ 15 16 17 ] , and has been proposed to impinge on the MEN both upstream and downstream of Tem1 [ 15 16 17 18 ] . During exit from mitosis, Cdc5 is required for Clb2 destruction, and similar to over-expression of Cdc14 [ 3 ] , induction of Cdc5 triggers precocious Clb2 degradation in pre-anaphase cells [ 19 20 ] . In addition to its role in exit from mitosis, Cdc5 has other functions during mitosis in budding yeast.
In pre-anaphase cells, Cdc14 is confined to the nucleolus as a subunit of the RENT complex (which also contains Net1/Cfi1 and Sir2), and its phosphatase activity is inhibited by Net1 [ 8 9 10 11 ] . Damaged DNA and mis-oriented mitotic spindles delay exit from mitosis (reviewed in [ 12 ] ), but after errors have been rectified or bypassed, Lte1, Nud1, and possibly other unidentified factors [ 13 ] promote Tem1 activation.
, submitted), Mob1 binds an essential protein kinase involved in spindle pole body duplication [ 27 ] , and Tem1, Cdc15, Dbf2, and Mob1 have all been implicated in cytokinesis ( [ 28 29 30 31 ] ; reviewed in [ 1 ] . The tab mutants provide a unique opportunity to address this issue.
Patterns of mitochondrial abnormalities revealed by transmission electron microscopy (TEM) suggest a model of vacuole formation by extension of mitochondrial outer membrane and expansion of the intermembrane space (Fig.
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