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Example sentences for: tem

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Wild type or tab3-1 derivatives of tem1Δ::GAL1-UPL-TEM1 were grown in galactose medium ( TEM1 induced), arrested in G1 with α factor, and released into glucose-containing medium to repress TEM1 expression (time 0).
Thirty-six percent represents a minimum estimate of TEM1 bypass efficiency in tab3-1 cultures, since cells with 1-2 cell bodies could have arisen from bypass events followed by successful cell separation.
tem1Δ by enabling Tem1-independent degradation of Clb2 and accumulation of Sic1.
This further suggested that the GFP-Nup mislocalization and TEM membrane proliferation were the consequence of the same process.
Biochemical and cell biological experiments indicate that Net1 acts as part of a complex named RENT that tethers Cdc14 to the nucleolus and inhibits Cdc14 phosphatase activity, and that CDC15 and TEM1 are required for the release of Cdc14 from Net1 at the end of mitosis [ 15 16 17 43 ] . Consistent with this notion, both net1 tab 2-1(which encodes a mutant version of Net1 with presumably reduced affinity for Cdc14) and CDC14 TAB 6-1(which encodes a mutant version of Cdc14 with reduced affinity for Tab2/Net1) bypass cdc15Δ [ 15 18 ] . Both net1 tab 2-1and CDC14 TAB 6-1impinge directly on mitotic exit, suggesting that other TAB genes may encode physiological regulators and effectors of the Mitotic Exit Network.