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Example sentences for: temecula
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The protein was soluble, and was purified by standard Ni-chelation chromatography [ 45 ] . Purified recombinant profilin and antibodies against profilin, RSV P protein, and F protein have been described previously [ 11 25 ] . Polyclonal antibodies to RSV proteins G, N, and M were purchased from Chemicon International (Temecula, CA) and the corresponding rhodamine-conjugated secondary antibody from Sigma.
Primary antibodies specific for the following proteins were used: monoclonal mouse anti-CD36 antibody, clone FA 6–152 (IgG) (Immunotech, Fullerton, California, United States), clone SMO (IgM) (Santa Cruz Biotechnology, Santa Cruz, California, United States), rabbit polyclonal anti-CD36 (Santa Cruz Biotechnology), rabbit polyclonal anti-aquaporin1, anti-aquaporin2, anti-Na/K/2Cl (Chemicon, Temecula, California, United States), rabbit polyclonal phospho38/MAPK and mouse monoclonal p38 (Cell Signaling Technology, Beverly, Massachusetts, United States), rabbit polyclonal p-src (Y418) (Biosource, Camarillo, California, United States), and mouse monoclonal anti-tubulin (Sigma, St. Louis, Missouri, United States).
Following cell thaw, NSs were expanded in suspension culture, and in each case were used between passages 5 and 7. Undifferentiated human NS cells (stage 1) were cultured and expanded in medium containing X-VIVO15 (Cambrex, Walkersville, Maryland, United States), N2 supplement (Invitrogen, Carlsbad, California, United States), 2 μg/ml heparin, 10 ng/ml leukemia inhibitory factor (Chemicon, Temecula, California, United States), 20 ng/ml epidermal growth factor (R&D Systems, Minneapolis, Minnesota, United States), and 20 ng/ml fibroblast growth factor-2 (R&D Systems), and they were frozen after enzymatic dissociation.
The cells were stained with a FITC labelled antibody directed against the major immediate early antigen (MIE) (Chemicon, Temecula, CA) and visualized using a fluorescent microscope.
Collagen and elastin deposition in cell and matrix layers was determined by solid-phase ELISA with antibodies against goat anti-collagen I (Chemicon, Temecula, CA, USA), mouse anti-collagen III (Telios, San Diego, CA, USA), mouse anti-collagen VI (Telios), or mouse anti-elastin (Sigma), at dilutions recommended by the manufacturers.
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