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Example sentences for: temecula
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Polyclonal antibodies used were MyoD (Santa Cruz Biotechnology, Santa Cruz, California, United States) and nestin (gift from R. McKay); monoclonal antibodies were vimentin, alpha smooth muscle actin, fast-switch myosin, pan-cytokeratin (all from Sigma), and human nuclear antigen (Chemicon, Temecula, California, United States).
Collagen and elastin deposition in cell and matrix layers was determined by solid-phase ELISA with antibodies against goat anti-collagen I (Chemicon, Temecula, CA, USA), mouse anti-collagen III (Telios, San Diego, CA, USA), mouse anti-collagen VI (Telios), or mouse anti-elastin (Sigma), at dilutions recommended by the manufacturers.
Primary antibodies used: mouse anti-chicken Coll I, Coll III, FN, and TN (Hybridoma Bank, Iowa), rabbit anti-chicken TN (Chemicon, Temecula CA).
The protein was soluble, and was purified by standard Ni-chelation chromatography [ 45 ] . Purified recombinant profilin and antibodies against profilin, RSV P protein, and F protein have been described previously [ 11 25 ] . Polyclonal antibodies to RSV proteins G, N, and M were purchased from Chemicon International (Temecula, CA) and the corresponding rhodamine-conjugated secondary antibody from Sigma.
The blots were first blocked with 5% non-fat dry milk in TPBS buffer (PBS with 0.5% Tween 20) at RT for 1 hr, and then incubated with 1:3000 dilution of anti-β-actin (Sigma, St. Louis MO), 0.75 μg/ml of anti-NR1 (Chemicon, Temecula, CA), 1:1000 dilution of anti-NR2A (Chemicon), 0.35 μg/ml of anti-NR2B (Chemicon) and 1 μg/ml of anti-GluR1 (Chemicon) antibodies, respectively, at 4°C overnight.
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