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Example sentences for: ten
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NTPDases do not form phosphorylated intermediates during catalysis, a conclusion also supported by lack of vanadate sensitivity and Pi product inhibition [ 3 4 5 6 ] . The catalytic reaction appears to be irreversible and no partial reactions have been observed [ 7 8 ] . Divalent cations like Ca 2+or Mg 2+are required for activity, and maximal activity is reached when the concentrations of substrates and divalent cations are equal [ 1 ] . The specific activities of NTPDases vary over a broad range from ten thousand units for potato apyrase to less than one hundred units for chicken gizzard ecto-ATPase [ 9 10 ] . Sequence comparisons indicate that most of NTPDases contain five highly conserved regions, apyrase conserved region, ACR1 - ACR5 [ 9 11 ] . However, the catalytic sites have not been identified, although ACR1 and ACR4 have been implicated in β- and γ-phosphate binding, respectively [ 9 ] .
He issued two Presidential Decision Directives, numbers 62 and 63, that built on the assignments to agencies that had been made in Presidential Decision Directive 39; laid out ten program areas for counterterrorism; and enhanced, at least on paper, Clarke's authority to police these assignments.
To validate sequences extracted by genomic database mining, all hOR gene coding sequences described in this paper were cloned by direct genomic PCR using mixed DNA from ten individuals as a template and oligonucleotide primers corresponding to the amino- and carboxy-terminal sequences of the corresponding ORFs.
However, three to ten bacterial control probes were designed to hybridize to each bacterial transcript.
In contrast, the P. medicaginis -infected library was prepared ten days after infection and individual plants varied in their degree of susceptibility (C.
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