Example sentences for: substrate-independent

How can you use “substrate-independent” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • These complexes bear ubiquitin ligase activity in a substrate-independent in vitro assay (Fig.

  • To determine whether Pcu3p is associated with such an activity and how it is affected by CSN, we developed a substrate-independent in vitro polyubiquitylation assay, similar to that originally reported by Lyapina et al.

  • Since the substrates of putative SCF Pop1pand SCF Pop2pubiquitin ligases are unknown, we adopted a substrate-independent assay originally described by Lyapina et al . [ 27 ] . For this experiment, we again used the strains harboring genomically integrated Myc epitope-tagged Pop1p or Pop2p in a background deficient in the respective heterooligomerization partner ( Δpop2 pop1-13myc , Δpop1 pop2-13myc strains).

  • Substrate-independent in vitro ubiquitylation activity was determined by immunoprecipitating Pop1p-13Myc or Pop2p-13Myc with Myc antibodies.


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