Words similar to substrate-binding
Example sentences for: substrate-binding
How can you use “substrate-binding” in a sentence? Here are some example sentences to help you improve your vocabulary:
The serine substrate-binding region is highly conserved, as is a monovalent cation (MVC) binding region [ 7] coordinating with G232, F/Y306, and G/A/S308.
Of these, the small residues preceding strands 3 and 6 contribute a positive φ angle, which results in turning of the C-α backbone to stabilize the β-barrel [ 62 ] . These shared features notwithstanding, the ancient DPBBs have no conserved residues in their catalytic or substrate-binding sites.
In L1, the residue at position 224 is a serine [ 35 ] , and the substrate-binding model for L1 predicts that this serine residue interacts with the carboxylate on substrate via a water molecule [ 37 ] . To test the proposed role of Ser224 in L1, serine was changed to an alanine (S224A), aspartic acid (S224D), and lysine (S224K), and these mutants were characterized using metal analyses, CD spectroscopy, steady-state kinetics, and pre-steady state kinetic studies.
The substrate-binding model showed that Tyr228 in L1 was position-conserved with Asn233 in the other crystallographically characterized metallo-β-lactamases [ 37 42 44 45 46 ] . Spencer and coworkers postulated that Tyr228 is part of an oxyanion hole that interacts with the β-lactam carbonyl on substrate and helps to stabilize the putative tetrahedral intermediate formed during substrate turnover [ 37 ] . To test this hypothesis, Tyr228 was changed to an alanine and to a phenylalanine to afford the Y228A and Y228F mutants, respectively.
The lack of conserved motifs in the substrate-binding region is consistent with the comparison of the E. coli and human GshB structures, which shows remarkably little similarity in this region of the protein.
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