Words similar to substrate
Example sentences for: substrate
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Whereas the positive control (trypsin) was able to digest the substrate at specific sites in the gel, the supernatants of our treated fibroblasts did not.
The soluble material was separated by centrifugation (3000 × g , 10 min) and assayed for absorbance at 235 nm to measure the concentration of product Δ 4-hexuronate (Molar extinction coefficient = 5500; [ 33 ] ). For the chondroitinase ABC reactions, each chondroitin sulfates A, B and C substrate (1 mg each) and concentrated CM, were incubated with 0.12 units of chondroitinase ABC in 1 ml of 50 mM Tris-HCl pH 8.0, 60 mM sodium acetate, and 0.02% BSA at 37°C.
We transfected v-crk alone or along with the chimera of the attenuated Src kinase domain fused to the C-terminal crk interacting region of the Cas substrate domain (Src*/Cas(3'SD), carrying the YDXP motifs) into Cos-7 cells and immunoprecipitated either the chimera or v-crk and assayed for interacting tyrosine phosphorylated proteins (Fig.
These findings indicated that cell cycle-dependent substrate degradation is unlikely to be controlled at the level of SCF Pop1p-Pop2pcomplex formation.
When HPB-ALL cells are plated onto β1 integrin substrate such as fibronectin, vascular cell adhesion molecule-1 (VCAM-1), or even antibodies to α4β1 integrins, they respond by extending pseudopodia and displaying a highly flattened, spread morphology (figure 3, panels 4, 7, and 10, and ref [ 32 ] ). However, electroporation of C3 appeared to alter HPB-ALL morphology resulting in the extension of very fine branched finger-like cellular processes when cells were plated on the β1 integrin substrates Fn (figure 3, panel 6), VCAM-1 (figure 3, panel 9), or a mAb specific for β1 integrins (figure 3, panel 12).
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