Example sentences for: substrate

How can you use “substrate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Alkaline phosphatase activity in the supernatant was determined spectrophotometrically at 405 nm wavelength as the amount of p-nitrophenol released from the substrate p-nitrophenyl phosphate (ALP10, Sigma).

  • In addition to buffering the redox status of the cytoplasm and protecting biomolecules against oxidative damage, GSH provides reducing equivalents to several enzymes (including ribonucleotide reductase [ 5], 3'-phosphoadenosine 5-phosphosulfate reductase [ 6] and arsenate reductase [ 7]), and serves as a substrate for glutathione- S -transferases, which detoxify potentially dangerous electrophiles.

  • However, in L1, Asn233 is 14 Å away from the modeled position of the substrate β-lactam carbonyl [ 37 ] . To test the role of Asn233 in substrate binding, the Asn was changed to a leucine (N233L) and to an aspartic acid (N233D), and these mutants were characterized by using metal analyses, CD spectroscopy, steady-state kinetics, and pre-steady state kinetic studies.

  • From an evolutionary perspective, the function of proteins within a protein family encompasses both variation, for example, substrate specificity reflected in amino acids lining substrate-binding pockets, and conservation, for example, regions responsible for general enzymatic activity or binding of a common molecular scaffold.

  • After centrifugation at 10,000 rpm for 10 min, 20 μl of the supernatant was added to 100 μl of luciferase assay substrate (Promega, The Netherlands).


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