Words similar to substrates

Example sentences for: substrates

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Purified TOP1 was pre-incubated with GST or GST-BTBD2 for 20 min before addition of the DNA substrate, which was either an end-labeled 36 bp fragment containing a single strong TOP1 cleavage site [ 24 ] , or a 161 bp PvuII-HindIII fragment of pBluescript (Stratagene) [ 25 ] ). End labeling of the DNA substrates was as previously described [ 24 25 ] . Cleavage reactions were continued for 20 min before stopping with 0.5% SDS.
In cells expressing kinase inactive Btk:ER, phosphorylation of Btk:ER was not detected and cellular substrates were not inducibly phosphorylated in response to 4-HT (Fig 1C).
With all substrates tested, the N233L and N233D mutants exhibited K m values that differed less than a factor of 4 than that observed for wild-type L1 (Tables 3, 4, 5).
Little is known about the function and substrates for CPKs in Arabidopsis.
The ubiquitination of proteins is regulated through precise selection of protein substrates by specific E3 ubiquitin ligases (Pickart 2001).