Example sentences for: subcloning

How can you use “subcloning” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • pVL-GST-NFATp was created by subcloning a Nde I - EcoR I fragment containing the NFATp cDNA from pBS-KS +-NFATp(NdeI) into the Nde I and EcoR I sites of pVL1392-GST (gift of S. Ruppert and R. Tjian).

  • These primers match the coding sequence of the Thermoplasma acidophilum A-ATPase A subunit (gene identification number 9369337) and introduce restriction sites useful in subcloning.

  • A pVL1392-HAX-NFATp(331-722) was made by subcloning a Nde I - Sma I fragment from pGEX-NFATp(331-722) (J.

  • The plasmids used to generate in situ probes were derived by subcloning an aliquot of the PCR product that was printed onto the microarray with Topo TA cloning (Invitrogen).

  • The five best reactive and specific clones, which were selected after subcloning, belonged to IgM class.


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