Words similar to subcloning
Example sentences for: subcloning
How can you use “subcloning” in a sentence? Here are some example sentences to help you improve your vocabulary:
PCR and standard subcloning procedures were use to create the initial transposon,
(full sequence at: http://momotion.med.yale.edu). The coding regions were tagged with a C -terminal green fluorescent protein (GFP) sequence by subcloning into the EcoR 1 site of the pEGFP-N1 vector (Clontech, Palo Alto, CA).
PCR amplicons of approximately 500 base-pairs (bp) were amplified from an Arabidopsis cDNA library constructed from leaf tissue or genomic DNA using sense and antisense gene-specific primers flanked by a Hind III and Sac I adaptor sites for subcloning, respectively.
The plasmids used to generate in situ probes were derived by subcloning an aliquot of the PCR product that was printed onto the microarray with Topo TA cloning (Invitrogen).
The five best reactive and specific clones, which were selected after subcloning, belonged to IgM class.