Example sentences for: subcloning

How can you use “subcloning” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • These primers match the coding sequence of the Thermoplasma acidophilum A-ATPase A subunit (gene identification number 9369337) and introduce restriction sites useful in subcloning.

  • The mammalian expression constructs of Smad1 and its deletion mutant Smad1C (271-465) were made by subcloning Smad1 into two modified pCMV6 vectors, which have either the Flag epitope (F-pCMV6) or the T7 epitope (T7-pCMV6) placed upstream of an array of new multiple cloning sites.

  • PCR and standard subcloning procedures were use to create the initial transposon, (full sequence at: http://momotion.med.yale.edu).

  • The coding regions were tagged with a C -terminal green fluorescent protein (GFP) sequence by subcloning into the EcoR 1 site of the pEGFP-N1 vector (Clontech, Palo Alto, CA).

  • Subcloning ORFs into pHIP and pBPhin


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