Words similar to subcloning
Example sentences for: subcloning
How can you use “subcloning” in a sentence? Here are some example sentences to help you improve your vocabulary:
PCR and standard subcloning procedures were use to create the initial transposon,
(full sequence at: http://momotion.med.yale.edu). The GFP-Siah1a-CT construct was made by subcloning the 3' end of Siah1a (from base 228) into pEGFP C1 in frame.
The five best reactive and specific clones, which were selected after subcloning, belonged to IgM class.
GDT was responsible for DNA subcloning, northern blot analysis, DNA sequencing, and BLAST algorithm analysis.
pBS-KS +-NFATp was generated by digesting pREP-NFATp with BssH II and Dra I, filling in the ends with Klenow, and ligating the resulting fragment into the Sma I site of pBS-KS +. pVL1392-HAX-NFATp was generated by subcloning a fragment excised from pBS-KS +-NFATp with Spe I and EcoR I into pVL1392-HAX (gift of S. Ruppert and R. Tjian) digested with Spe I and EcoR I. pBS-KS +-NFATp(NdeI), which has an Nde I site, containing the NFATp translational start site ATG, was generated in two steps.