Example sentences for: subcloning

How can you use “subcloning” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The mammalian expression constructs of Smad1 and its deletion mutant Smad1C (271-465) were made by subcloning Smad1 into two modified pCMV6 vectors, which have either the Flag epitope (F-pCMV6) or the T7 epitope (T7-pCMV6) placed upstream of an array of new multiple cloning sites.

  • Subcloning of NRG1 constructs

  • Subcloning ORFs into pHIP and pBPhin

  • EE-tagged sα i2 was constructed by subcloning a EcoRI-BglII fragment from EE-α i2 -pcDNAI into sα i2 -pcDNAI.

  • pVL-GST-NFATp was created by subcloning a Nde I - EcoR I fragment containing the NFATp cDNA from pBS-KS +-NFATp(NdeI) into the Nde I and EcoR I sites of pVL1392-GST (gift of S. Ruppert and R. Tjian).


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