Example sentences for: subcloning

How can you use “subcloning” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR and standard subcloning procedures were use to create the initial transposon, (full sequence at: http://momotion.med.yale.edu).

  • The GFP-Siah1a-CT construct was made by subcloning the 3' end of Siah1a (from base 228) into pEGFP C1 in frame.

  • The five best reactive and specific clones, which were selected after subcloning, belonged to IgM class.

  • GDT was responsible for DNA subcloning, northern blot analysis, DNA sequencing, and BLAST algorithm analysis.

  • pBS-KS +-NFATp was generated by digesting pREP-NFATp with BssH II and Dra I, filling in the ends with Klenow, and ligating the resulting fragment into the Sma I site of pBS-KS +. pVL1392-HAX-NFATp was generated by subcloning a fragment excised from pBS-KS +-NFATp with Spe I and EcoR I into pVL1392-HAX (gift of S. Ruppert and R. Tjian) digested with Spe I and EcoR I. pBS-KS +-NFATp(NdeI), which has an Nde I site, containing the NFATp translational start site ATG, was generated in two steps.


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