Example sentences for: subcloning

How can you use “subcloning” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The GFP-Siah1a-CT construct was made by subcloning the 3' end of Siah1a (from base 228) into pEGFP C1 in frame.

  • Cloning, subcloning, and gene replacement for the expression of HA-Ppt1p in yeast cells

  • The GAL4 fusion plasmids were obtained by subcloning the appropriate cDNAs into the pGaL4PolyII plasmid [ 24 ] , in-frame with the yeast GAL4 binding domain coding sequence.

  • 45-263) followed by subcloning the PCR products into the EcoR1/Xho1 sites of the modified T7-pCMV6 vector.

  • PCR amplicons of approximately 500 base-pairs (bp) were amplified from an Arabidopsis cDNA library constructed from leaf tissue or genomic DNA using sense and antisense gene-specific primers flanked by a Hind III and Sac I adaptor sites for subcloning, respectively.


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