Words similar to subcloning

Example sentences for: subcloning

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The mammalian expression constructs of Smad1 and its deletion mutant Smad1C (271-465) were made by subcloning Smad1 into two modified pCMV6 vectors, which have either the Flag epitope (F-pCMV6) or the T7 epitope (T7-pCMV6) placed upstream of an array of new multiple cloning sites.
The Smad1 (G419S) mutant was a kind gift from J. Wrana [ 40 ] . The subcloning of Smad1 (G419S), Smad1NL (1-271), Smad1L (147-271) into pEG202 were all carried out similarly by the PCR approach.
N3-F pCMV6 was made by PCR amplification of HsN3 (1-263) followed by subcloning the PCR products into the EcoR1/EcoRV sites of pFlag-CMV5C vector (Kodak).
The GAL4 fusion plasmids were obtained by subcloning the appropriate cDNAs into the pGaL4PolyII plasmid [ 24 ] , in-frame with the yeast GAL4 binding domain coding sequence.
pVL-GST-NFATp was created by subcloning a Nde I - EcoR I fragment containing the NFATp cDNA from pBS-KS +-NFATp(NdeI) into the Nde I and EcoR I sites of pVL1392-GST (gift of S. Ruppert and R. Tjian).