Example sentences for: subclones

How can you use “subclones” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Starting materials were the previously cloned genomic subclones and a plasmid carrying EGFP-F (pEGFP-F from Clontech, Palo Alto, CA).

  • BRCA1 antisense subclones demonstrated other traits associated with a tumorigenic phenotype, such as the ability to grow in soft agar independent of estrogen, whereas control clones could only form colonies with the addition of estrogen.

  • The exon-containing regions of hybridizing subclones were sequenced and the genomic sequences compared with the cDNA sequences to identify transcription start sites and exon/intron junctions.

  • For example, the transcript levels of 310 of these genes changed by 1.5-fold or more in MDA PCa 2a and 2b cells following 48 hours of exposure to R1881; transcripts for 262 genes changed by 1.5-fold or greater in single treatments of all four LNCaP androgen-deprived subclones; and transcripts for 81 genes changed by 1.5-fold or greater in the three LAPC-4 and LAPC-4 (androgen-deprived) array experiments.

  • BRCA1 RNA and protein levels were successfully reduced in pooled and isolated subclones of antisense-infected populations of BG-1 cells.


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