Words similar to subcloned
Example sentences for: subcloned
How can you use “subcloned” in a sentence? Here are some example sentences to help you improve your vocabulary:
The largest clone overlapping all positive hits was sheared and subcloned into 3-kb plasmids, and sequenced using methods described in [ 9].
In order to characterize the PfPP1 cDNA we subcloned it into pET-15b such that the protein is expressed with an N-terminal (His) 6 -tag.
The resulting fragment (hCARΔcyt) was restriction endonuclease digested and subcloned into the EcoRI/SmaI site of the VA human CD2 mini-gene containing the human CD2 promoter and 3' LCR [ 16 ] . Automated sequencing (Applied Biosystems, Foster City, CA; University of Alabama at Birmingham Department of Microbiology sequencing facility) of the entire construct confirmed its accuracy.
Research Genetics, Inc.) with RSAI and then subcloned the DNA fragments into pZero 2.0 according to the manufacturer's instructions.
To make pJC3, we subcloned the Nsi I- Pst I fragment (1246 bp) of pTU23 [ 35] into the Pst I site of pPD118.
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