Example sentences for: subcloned

How can you use “subcloned” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • This fragment was subcloned into the HindIII site of the EGFP vector by plus/minus green cloning.

  • The resulting DNA fragment was purified by agarose gel electrophoresis, cleaved with Eco R I, and subcloned into pBluescript KS-.

  • To generate p102, the coding region of Bex1 GFP [ 15 ] was subcloned into pBS-MyBam [ 33 ] . The fusion of GFP to the N-terminal region of the myosin-II heavy chain gene was subsequently subcloned into pBigMyD.

  • Sequencing of subcloned genomic fragments and comparison with the cDNA sequence allowed us to construct an exon map of the NPM3 gene (Figure 1A).

  • The colonies were lysed and the subcloned fragments were reamplified with the same primers.


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