Example sentences for: subcloned

How can you use “subcloned” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The purified DNA template was bacterially subcloned followed by plasmid isolation and sequencing.

  • pJM127 was digested with Eco RI and Xba I to release a pcrG fragment that was subsequently subcloned into EcoRI- and XbaI-cleaved pBAD18-Kan to create pJM132.

  • A 240 bp DNA fragment from S. cerevisiae ARS1 chromosomal DNA replication origin containing all the key elements including boxes A, B1, B2 and B3 was subcloned in pBlueScript KS(+) between Eco RI and Hin dIII.

  • [ 30 ] and by us [ 31 ] . Human RGS3 cDNA [ 24 ] was restricted from the pRC/CMV vector with EcoRI, and then subcloned into the corresponding site of the pACCMVpLpa plasmid.

  • Wild type p53 or p53 bearing mutations in serines 33 or 37 were subcloned into pGEX-2T GST fusion vector (Pharmacia, NJ) and p53-GST purified as previously described [ 33].


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