Example sentences for: subcloned

How can you use “subcloned” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Gal-GRIP1 NR box (1,2,3) fusion (amino acids 610-770) was prepared by PCR amplification of the appropriate region of GRIP1 (primers obtained from Biomolecular Resource Center, UCSF) containing EcoRI and SalI sites, the PCR fragment was digested with these enzymes and subcloned into the pM GAL4 expression vector (Clontech Laboratories, Inc.

  • The plasmid pTK2 contains HSV-2 SB5 sequence coordinates 44,469-48,184 subcloned into the HindIII / EcoRI site of pUC19.

  • PCR products were subcloned into TOPO™ TA cloning vectors (Invitrogen) and subjected to cycle sequencing (Division of Molecular Transport, Department of Internal Medicine, University of Texas Southwestern Medical Center).

  • For the yeast two-hybrid screen, full-length Smad1 was subcloned into BamH1/Not1 sites of PEG202 by a PCR method, resulting in the inframe fusion between the LexA DNA binding domain (1-202) and Smad1.

  • This fragment was subcloned into the HindIII site of the EGFP vector by plus/minus green cloning.


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