Example sentences for: subcloned

How can you use “subcloned” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Amplification products were subcloned into the T-vector (Promega) and sequenced.

  • This frame-shift mutation was re-ligated into an otherwise full-length α 1S , subcloned into the mammalian expression vector pSG5, and transfected into dysgenic (α 1S null) myotubes.

  • Gal-GRIP1 NR box (1,2,3) fusion (amino acids 610-770) was prepared by PCR amplification of the appropriate region of GRIP1 (primers obtained from Biomolecular Resource Center, UCSF) containing EcoRI and SalI sites, the PCR fragment was digested with these enzymes and subcloned into the pM GAL4 expression vector (Clontech Laboratories, Inc.

  • RACE products were then subcloned into pbluescript KS II +using TA vector cloning.

  • The largest clone overlapping all positive hits was sheared and subcloned into 3-kb plasmids, and sequenced using methods described in [ 9].


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