Example sentences for: subcloned

How can you use “subcloned” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A gene encoding a plant-optimized GFP variant, mGFP5 [ 24], was placed in frame with a LacI -SV40 nuclear localization signal fusion ( GFP-LacI/NLS ) [ 25] and then subcloned into the vector pTA7002 under the control of the glucocorticoid receptor (GR)-responsive promoter near the left border.

  • Complementary DNAs were amplified by PCR, subcloned into pREp81.

  • Development of our control set of 10 Arabidopsis oligonucleotides and corresponding set of PCR amplicons subcloned into pSP64poly(A) serve as a valuable quality-control resource for cDNA/oligo microarrays.

  • This sequence was then subcloned into the XhoI and BamHI sites of the pET-15b vector by designing similar primers but containing the respective restriction sites.

  • In order to characterize the PfPP1 cDNA we subcloned it into pET-15b such that the protein is expressed with an N-terminal (His) 6 -tag.


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