Words similar to subcloned
Example sentences for: subcloned
How can you use “subcloned” in a sentence? Here are some example sentences to help you improve your vocabulary:
The purified DNA template was bacterially subcloned followed by plasmid isolation and sequencing.
pJM127 was digested with Eco RI and Xba I to release a pcrG fragment that was subsequently subcloned into EcoRI- and XbaI-cleaved pBAD18-Kan to create pJM132.
A 240 bp DNA fragment from S. cerevisiae ARS1 chromosomal DNA replication origin containing all the key elements including boxes A, B1, B2 and B3 was subcloned in pBlueScript KS(+) between Eco RI and Hin dIII.
[ 30 ] and by us [ 31 ] . Human RGS3 cDNA [ 24 ] was restricted from the pRC/CMV vector with EcoRI, and then subcloned into the corresponding site of the pACCMVpLpa plasmid.
Wild type p53 or p53 bearing mutations in serines 33 or 37 were subcloned into pGEX-2T GST fusion vector (Pharmacia, NJ) and p53-GST purified as previously described [ 33].