Words similar to subconfluent
Example sentences for: subconfluent
How can you use “subconfluent” in a sentence? Here are some example sentences to help you improve your vocabulary:
Subconfluent cells (5 × 10 3cells/cm 2) were transfected with 2 μg of the relevant plasmid using Fugene-6 (Boehringer Mannheim) in DMEM containing 0.2% BSA, 100 U/ml penicillin, 100 μg/ml streptomycin, 0.292 mg/ml glutamine (serum-free medium) following the manufacturer's protocol.
As infections were most efficient in subconfluent Caco-2 cells, which do not have differentiated apical and basolateral surfaces, we chose these conditions for our microarray experiments with the goal of inducing as much actin-based motility as possible.
In light of recent developments in understanding the biology of Toll-like receptors, the NFκB-dominated responses we observe fit well within a model in which undifferentiated, subconfluent Caco-2 cells can recognize Gram-positive cell-surface moieties and induce what is termed an 'innate immune response'.
All infections were performed on subconfluent cells that were passaged between 7 and 10 times after arrival from ATCC (original stock was p18).
For microarray experiments, cells were maintained as a non-polarized, subconfluent population.
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