Words similar to subconfluent
Example sentences for: subconfluent
How can you use “subconfluent” in a sentence? Here are some example sentences to help you improve your vocabulary:
All infections were performed on subconfluent cells that were passaged between 7 and 10 times after arrival from ATCC (original stock was p18).
Nuclear isolation, transcription elongation reactions and hybridizations were performed as described previously with minor modifications [ 7 ] . Subconfluent H441 cells were incubated in serum-free media for 24 hours and then exposed to media plus either vehicle or insulin (2.
In a study of MP-release in subconfluent, confluent and overgrown HUVEC culture, the cells at 24 hrs (50% of confluence), 48 hrs (reaching confluence) or 72 hrs (overgrown) in culture were incubated for 24 hrs with 0.5 % DMSO, 500 nM or 5 μM CPT in medium.
To investigate whether maturation of the cell culture influences the degree of MP-release from HUVEC in response to CPT, we compared MP-release from subconfluent HUVEC (50% of confluence) at 24 hrs in culture, HUVEC reaching confluence at 48 hrs in culture and overgrown HUVEC at 72 hrs in culture.
Subconfluent cells (5 × 10 3cells/cm 2) were transfected with 2 μg of the relevant plasmid using Fugene-6 (Boehringer Mannheim) in DMEM containing 0.2% BSA, 100 U/ml penicillin, 100 μg/ml streptomycin, 0.292 mg/ml glutamine (serum-free medium) following the manufacturer's protocol.
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