Words similar to ribosylation
Example sentences for: ribosylation
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The phenotype induced by C3 mediated ribosylation of Rho in HPB-ALL could result from either aberrant retraction of recently formed pseudopods, or altered pseudopodial extension.
Although ribosylation of other Rho family proteins such as Rac1 can occur with C3 [ 33 ] , this seemed to be very weak [ 13 ] . In our work, C3 electroporated into the T cell line HPB-ALL caused complete ADP-ribosylation of Rho proteins (~25 kDa).
After electroporation, cells were transferred into 1.5 ml eppendorf tubes and incubated for 1 hr at 37°C to allow ribosylation.
ADP ribosylation factors (ARF) and Rho (reviewed in [ 7 8 ] ). Regulation of PLD2 is less well understood.
By this method, any further ADP ribosylation of Rho can be identified in vitro after in vivo ribosylation, thus serving as a measure of the efficiency of the electroporation procedure for introducing C3 exoenzyme into HPB-ALL cells [ 31 ] . As figure 1Bdemonstrates, electroporation of C3 transferase (Figure 1B, lane 3) prior to cell disruption prevented further ribosylation of Rho proteins in the cell lysates.