Words similar to ribosylation

Example sentences for: ribosylation

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After thorough washing, the cells were incubated for 1 hour at 37°C to allow in vivo ribosylation, then sonicated and lysates subjected to further C3 ribosylation by the addition of 2 μCi [ 32P]-NAD and 1 μg of C3 in ribosylation buffer.
After electroporation, cells were transferred into 1.5 ml eppendorf tubes and incubated for 1 hr at 37°C to allow ribosylation.
Although Rac1 and Cdc42 were identified in western blots of whole cell lysates prepared from the T cell lines HPB-ALL, CEM, HSB (data not shown), and PB human T lymphocytes (data not shown), there was no detection of ribosylation in the lower molecular weight Rac and Cdc42 related GTPases in HPB-ALL cells, indicating the specificity of C3.
By this method, any further ADP ribosylation of Rho can be identified in vitro after in vivo ribosylation, thus serving as a measure of the efficiency of the electroporation procedure for introducing C3 exoenzyme into HPB-ALL cells [ 31 ] . As figure 1Bdemonstrates, electroporation of C3 transferase (Figure 1B, lane 3) prior to cell disruption prevented further ribosylation of Rho proteins in the cell lysates.
Thus electroporation of the exoenzyme C3 fully ribosylates Rho in vivo , to the extent that no further in vitro ribosylation of Rho can be detected after cell lysis.