Words similar to ribosylation

Example sentences for: ribosylation

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By this method, any further ADP ribosylation of Rho can be identified in vitro after in vivo ribosylation, thus serving as a measure of the efficiency of the electroporation procedure for introducing C3 exoenzyme into HPB-ALL cells [ 31 ] . As figure 1Bdemonstrates, electroporation of C3 transferase (Figure 1B, lane 3) prior to cell disruption prevented further ribosylation of Rho proteins in the cell lysates.
After thorough washing, the cells were incubated for 1 hour at 37°C to allow in vivo ribosylation, then sonicated and lysates subjected to further C3 ribosylation by the addition of 2 μCi [ 32P]-NAD and 1 μg of C3 in ribosylation buffer.
Although ribosylation of other Rho family proteins such as Rac1 can occur with C3 [ 33 ] , this seemed to be very weak [ 13 ] . In our work, C3 electroporated into the T cell line HPB-ALL caused complete ADP-ribosylation of Rho proteins (~25 kDa).
After electroporation, cells were transferred into 1.5 ml eppendorf tubes and incubated for 1 hr at 37°C to allow ribosylation.
ADP ribosylation factors (ARF) and Rho (reviewed in [ 7 8 ] ). Regulation of PLD2 is less well understood.