Words similar to ribonuclease
Example sentences for: ribonuclease
How can you use “ribonuclease” in a sentence? Here are some example sentences to help you improve your vocabulary:
The N-terminal core region of high similarity between family members correlates to a region in nucleophosmin/B23 that has been shown to be involved in oligomerization as well as chaperone activity [ 44 ] . A central portion between nucleophosmin's two acidic domains is required for ribonuclease activity [ 44 ] ; this region does not have a corresponding domain in either NPM3 or nucleoplasmin, suggesting that these proteins would lack such activity.
For the ribonuclease protection assays, probes were made using the MAXIscript kit (Ambion, Austin, TX), 32P-CTP, and T7 RNA polymerase.
Confirmation that resisitant clones expressed IDO antisense RNA was obtained by isolating total RNA from G418 resistant clones, treating with ribonuclease free DNase RQ1 (Promega) and reverse transcribing RNA into cDNA in the presence of an IDO sense primer [ 17].
During synthesis of the minus-strand DNA the pregenomic RNA is degraded by the ribonuclease H (RNAseH) activity of P. However, the RNAseH activity does not digest the extreme 5' end of the template RNA, and this ∼ 18 nucleotide capped RNA fragment is then transferred to direct repeat 2 (DR2), where it acts as a primer for synthesis of plus-strand DNA [ 11, 12].
Ribonuclease protection assay
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