Example sentences for: ribonuclease

How can you use “ribonuclease” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Ribonuclease protection assays

  • We have previously investigated nmt55/p54 nrbprotein expression in human breast tumors and observed a statistically significant association between decreased nmt55/p54 nrbexpression, loss of hERα and hPR and increased tumor size [ 13 ] . It is possible that decreased nmt55/p54 nrbexpression represents poor tumor differentiation, increased tumor growth and metastases since reduced or absent hERα expression and function correlates with poor tumor differentiation [ 11 36 ] and tumor size is a strong indicator of tumor progression [ 37 ] . Here we investigated nmt55/p54 nrbmRNA expression using Northern blot analyses and ribonuclease protection assays to determine if the reduction of nmt55/p54 nrbprotein expression was the result of transcriptional regulation.

  • Ribonuclease protection assay (RPA)

  • BRCA1 ribonuclease protection probe was made using an in vitro transcription kit (Ambion, Inc, Austin, TX, USA) as previously described [ 10] and derived clones were tested for protein levels by Western blot analysis using an anti-BRCA1 (Oncogene Research, Ab-1, Cambridge, MA, USA) antibody.

  • To test the possibility that nmt55/p54 nrbmay be transcriptionally downregulated in ER- tumors, we analyzed mRNA levels from a series of human breast tumors utilizing Northern blot analyses and ribonuclease protection assays.


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