Words similar to ribozymes
Example sentences for: ribozymes
How can you use “ribozymes” in a sentence? Here are some example sentences to help you improve your vocabulary:
For example, Herschlag and co-workers have previously shown that a nucleobase could complement an abasic site within the hammerhead ribozyme, restoring cleavage activity [ 1 ] . Similarly, Porta and Lizardi engineered a hammerhead ribozyme that assumed an inactive secondary structure; upon the addition of an oligonucleotide that disrupted the engineered secondary structure, the ribozyme was activated [ 2 ] . More recently, it has previously been shown that many selected nucleic acid binding species (aptamers) undergo conformational changes upon ligand binding [ 3 4 5 6 ] In addition, when aptamers are conjoined to ribozymes, the ligand-induced conformational changes in the aptamers frequently modulate the structures and hence the activities of the ribozymes [ 7 8 9 ] For example, binding of theophylline to the anti-theophylline aptamer [ 10 ] modulates the conformation of residue [ 11 12 ] and the overall stability of the aptamer [ 10 13 ] . Appending the anti-theophylline aptamer to the hammerhead ribozyme allowed cleavage activity to be modulated by 110-fold by theophylline [ 13 ] . Similarly, appending the anti-theophylline aptamer to a small ribozyme ligase (L1) generated an aptazyme that was activated 1,600-fold by saturating theophylline concentrations [ 14 ] .
Sirna began as the biotech startup Ribozyme Pharmaceuticals, which tried to develop ribozymes to treat several conditions, including hepatitis C. A ribozyme is an RNA molecule whose sequence and structure allow it to cleave specific target RNA molecules (see Figure 1).
But does RNAi have a better chance to succeed as a drug than antisense or ribozymes?
The plotted data and rate constants for Th1P5 and Th2P6 constructs are shown in Figures 2band 2cand Table 2. The changes in the extent of reaction observed between individual experiments, and thus the attendant error bars, were consistent with those observed during the kinetic analyses of other ribozymes.
High levels of OPN in breast tumor cell lines appear to correlate with a more aggressive in vivo phenotype [ 2 20 ] . Transfection of OPN expression constructs into cells producing noninvasive mammary epithelial tumors has been reported to convert them to a malignant phenotype [ 8 21 ] . Furthermore, transfection of antisense OPN constructs or OPN-targeting ribozymes into transformed tumorigenic cells can result in impaired anchorage-independent growth, as well as in diminished tumor-forming ability in vivo [ 22 23 ] . OPN is also typically overexpressed in human tumors, including breast cancer [ 15 24 ] . However, the source of OPN in primary breast tumors was originally thought to be infiltrating macrophages rather than tumor cells themselves [ 15 ] .
Loading...