Example sentences for: ribozymes

How can you use “ribozymes” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The road to clinical success is littered with great ideas that have come a cropper along the way, including two other RNA-based therapies, antisense and ribozymes, both of which showed promise at the bench but have largely stumbled before reaching the bedside.

  • “There was a lot of clinical naïveté” in the early days of antisense and ribozymes, according to Nassim Usman, Vice President for Research and Development at Sirna Therapeutics in Boulder, Colorado.

  • In vivo studies have supported the conclusion that CBP/p300 are components of the hormonal-regulation of transcription in fibroblasts isolated from a p300-/- mouse; and loss of the p300 gene severely affects retinoic acid (RA)-dependent transcription [ 91 ] . In a separate study using hammerhead ribozymes that specifically cleave CBP or p300 mRNA, Kawasaki et al [ 92 ] reported that reduced cellular CBP or p300 levels resulted in compromised expression of endogenous RA-inducible genes such as p21/Waf1 and p27 cdk inhibitors.

  • Growing disillusionment with antisense and ribozymes coincided with the discovery of RNAi and the realization that it was a far more potent way to silence gene expression.

  • High levels of OPN in breast tumor cell lines appear to correlate with a more aggressive in vivo phenotype [ 2 20 ] . Transfection of OPN expression constructs into cells producing noninvasive mammary epithelial tumors has been reported to convert them to a malignant phenotype [ 8 21 ] . Furthermore, transfection of antisense OPN constructs or OPN-targeting ribozymes into transformed tumorigenic cells can result in impaired anchorage-independent growth, as well as in diminished tumor-forming ability in vivo [ 22 23 ] . OPN is also typically overexpressed in human tumors, including breast cancer [ 15 24 ] . However, the source of OPN in primary breast tumors was originally thought to be infiltrating macrophages rather than tumor cells themselves [ 15 ] .


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