Words similar to ribozymes
Example sentences for: ribozymes
How can you use “ribozymes” in a sentence? Here are some example sentences to help you improve your vocabulary:
The plotted data and rate constants for Th1P5 and Th2P6 constructs are shown in Figures 2band 2cand Table 2. The changes in the extent of reaction observed between individual experiments, and thus the attendant error bars, were consistent with those observed during the kinetic analyses of other ribozymes.
In vivo studies have supported the conclusion that CBP/p300 are components of the hormonal-regulation of transcription in fibroblasts isolated from a p300-/- mouse; and loss of the p300 gene severely affects retinoic acid (RA)-dependent transcription [ 91 ] . In a separate study using hammerhead ribozymes that specifically cleave CBP or p300 mRNA, Kawasaki et al [ 92 ] reported that reduced cellular CBP or p300 levels resulted in compromised expression of endogenous RA-inducible genes such as p21/Waf1 and p27 cdk inhibitors.
Sirna began as the biotech startup Ribozyme Pharmaceuticals, which tried to develop ribozymes to treat several conditions, including hepatitis C. A ribozyme is an RNA molecule whose sequence and structure allow it to cleave specific target RNA molecules (see Figure 1).
For example, Herschlag and co-workers have previously shown that a nucleobase could complement an abasic site within the hammerhead ribozyme, restoring cleavage activity [ 1 ] . Similarly, Porta and Lizardi engineered a hammerhead ribozyme that assumed an inactive secondary structure; upon the addition of an oligonucleotide that disrupted the engineered secondary structure, the ribozyme was activated [ 2 ] . More recently, it has previously been shown that many selected nucleic acid binding species (aptamers) undergo conformational changes upon ligand binding [ 3 4 5 6 ] In addition, when aptamers are conjoined to ribozymes, the ligand-induced conformational changes in the aptamers frequently modulate the structures and hence the activities of the ribozymes [ 7 8 9 ] For example, binding of theophylline to the anti-theophylline aptamer [ 10 ] modulates the conformation of residue [ 11 12 ] and the overall stability of the aptamer [ 10 13 ] . Appending the anti-theophylline aptamer to the hammerhead ribozyme allowed cleavage activity to be modulated by 110-fold by theophylline [ 13 ] . Similarly, appending the anti-theophylline aptamer to a small ribozyme ligase (L1) generated an aptazyme that was activated 1,600-fold by saturating theophylline concentrations [ 14 ] .
For construction of the recombinant adenovirus containing the M6P/IGF2R-ribozyme (pAd-GFP/Rz-IGF2R), the segments containing the ribozymes were amplified by PCR and cloned into a pAdTrack-CMV vector and then recombined homologously with an adenoviral backbone pAdEasy 1 vector to generate pAd-GFP/Rz-IGF2R, following the protocol described by He et al [ 30 ] . The pAd-GFP/Rz-IGF2R carries both the Rz-IGF2R and GFP (as reporter) genes, each under the control of separate cytomegalovirus (CMV) promoters.
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