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Example sentences for: rdna
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The release of Cdc14 from nucleolar Net1 promotes mitotic exit [ 15 17 18 ] . net1 tab 2-1cells display a panel of nucleolar defects including reduction of nucleolar silencing, mislocalization of nucleolar antigens, alteration of rDNA morphology, and reduction of rRNA synthesis [ 16 18 ] . To test whether perturbation of nucleolar structure is consistently associated with bypass of cdc15Δ, we immunolocalized the nucleolar antigen Nop1 in all tab mutants.
In Xenopus, biochemical analysis of replication and cell cycle events using egg extracts has helped to understand the mechanism of eukaryotic DNA replication [ 10 ] . However, two dimensional gel electrophoresis analysis of the rDNA locus showed that replication initiated at all sites tested [ 11 ] . All six human homologs of yeast and Drosophila ORC subunits have been cloned and characterized [ 12 13 ] . Other replication proteins like Cdc6, Cdt1, MCMs, Cdc45 that are essential for initiation of DNA replication have also been reported [ 12 ] . Conservation of replication factors among higher eukaryotes suggests that functionally they may play similar roles.
The PCR reaction mixture consisted of 1 × buffer A (Perkin-Elmer), 3 mM MgCl 2 , 200 μM dNTPs, 90 nM of each adenovirus primer, 100 nM of each 18S rDNA primer, 200 nM of each probe, 0.6 U AmpliTaq Gold polymerase (Perkin-Elmer) and 5 μl of total DNA sample.
The library used to test FastGroup consisted of 94 bacterial 16S rDNA obtained from an environmental sample.
These could include controls such as direct microscopic counts of active and inactive bacteria [ 19 ] , extraction and quantitation of ATP [ 23 ] , extraction and analysis of fatty acids [ 24 ] , real-time PCR of universal 16S rDNA markers [ 25 ] , and/or use of a larger variety of culture media, including dilute organic media, in place of or in addition to plate count agar [ 26 ] . All of these approaches also have problems, such as the possible presence in soil of biological molecules that are not associated with living cells, the always-present inability of microbiologists to culture most of the bacteria present in soil, and the often unpredictable performance of PCR primers.
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