Words similar to rarα
Example sentences for: rarα
How can you use “rarα” in a sentence? Here are some example sentences to help you improve your vocabulary:
SCC25 control, RARα overexpressing, and S77A RARα mutant clones were cultured on plastic then incubated with 10 μM BrdU for 1 hour.
By comparison, proliferation of clones expressing HA tagged RARα was 90% of G418 resistant control cells.
Previously we characterized another RARα mutant in which the receptor protein was truncated after amino acid 403, thereby deleting the AF-2 domain [ 11 ] . This truncated receptor exhibited similar properties to the S77A mutant (growth inhibition and repression of RA responsive gene expression including EGFR).
The human squamous cell carcinoma lines used in this study were purchased from the American Type Culture Collection and have been described previously [ 11 ] . Cells were cultured in Dulbecco's modified Eagle medium (DMEM), 10% fetal bovine serum (FBS), and 40 μg/ml gentamicin at 37°C in a humidified atmosphere of 5% CO 2 . S77A mutant or wild type RARα cDNAs were cloned into the pCruz-HA expression vector.
SCC25 cells expressing the S77A mutant RARα proliferated at markedly reduced rates compared to control clones (Fig.
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