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Example sentences for: ptgs
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In 1999, plant scientists studying PTGS provided a crucial clue when they detected small (approximately 25 nucleotide-long) RNAs corresponding to silenced target genes in transgenic plants (Hamilton and Baulcombe 1999).
Vice versa, this transition raises the question whether the originally strong transcription of the GFP-COP1 genes, or perhaps the ensuing PTGS phase, somehow sets the stage for the subsequent trans-silencing activity, especially if multimeric T-DNA loci are involved.
Polymerization of ribonucleoside triphosphates (NTPs), which is central to a variety of crucial biological processes, including transcription, primer synthesis during DNA replication, addition of polyA tails to mRNA, addition of CCA to tRNA, uridylation in RNA editing, replication of RNA viruses, amplification of RNA in eukaryotic post-transcriptional gene silencing (PTGS), and oligoadenylate synthesis during interferon signaling, is catalyzed by a broad variety of distinct enzymes [ 1 2 3 4 ] . These polymerases belong to two major mechanistic categories, those that copy a nucleic acid template and those that are independent of a template.
Transgene PTGS thus tapped into a preexisting natural mechanism for combating viruses.
Cosuppression of the endogenous COP1 gene is a consequence of posttranscriptional silencing, while transcriptionally trans-silenced loci do not cosuppress COP1 [ 27 ] . In our hands, a subset of four 35S:GFP-COP1 transgene loci shifted from a cosuppressing state, bona fide PTGS, to a non-cosuppressing state, i.e.