Example sentences for: ptc-

How can you use “ptc-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Early models of Hh signaling proposed a Hh-regulated Ptc-Smo complex that directly controlled the conformation of Smo, making endogenous ligands unnecessary.

  • TM3 cells expressing both epitope-tagged Ptc and Smo were derived by infecting first with an HA-Smo construct and subsequently with a Ptc-GFP construct.

  • PCR reactions and restriction digests (PCR-RFLP) are optimized for each marker and performed on the PTC-100 Programmable Thermal Controller (MJ Research) outfitted with a heated lid for oil-free amplifications.

  • We conducted the PCR reaction in a MJ Research (Watertown, Massachusetts) PTC-200 Peltier Thermal Cycler programmed with an initial cDNA synthesis and pre-denaturation step of 50°C for 30 minutes and 94°C for 2 minutes followed by then 36 cycles of denature, annealing and extension (15 s at 94°C, 30 s at 55°C, 30 s at 68°C).

  • The levels of Ptc were relatively low in these lines and a standard immunoprecipitation procedure followed by western blotting was required to detect the Ptc-GFP protein.


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