Example sentences for: ptc-

How can you use “ptc-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In contrast to the results with Ptc-GFP, incubation of cells with both Hh protein and the small-molecule agonist resulted in the apparent accumulation of HA-Smo protein after 5 hours of incubation.

  • To test whether similar phenomena occur in mammalian cells with Hh protein and agonist, we generated stable cell lines expressing two epitope-tagged proteins, Ptc coupled to green fluorescent protein, Ptc-GFP, and Smo coupled to a fragment of influenza hemagglutinin, HA-Smo.

  • We conducted the PCR reaction in a MJ Research (Watertown, Massachusetts) PTC-200 Peltier Thermal Cycler programmed with an initial cDNA synthesis and pre-denaturation step of 50°C for 30 minutes and 94°C for 2 minutes followed by then 36 cycles of denature, annealing and extension (15 s at 94°C, 30 s at 55°C, 30 s at 68°C).

  • Stable HA-Smo and Ptc-GFP lines were established by puromycin selection following infection of TM3 cells with the respective retroviruses.

  • The thermocycling conditions were as follows: 95°C for 3 minutes, then 30 cycles of 95°C for 45 seconds, 50°C for 30 seconds and 75°C for 60 seconds, then 75°C for 10 minutes, using a PTC-100 Thermocycler (M.J.


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