Words similar to ptc
Example sentences for: ptc
How can you use “ptc” in a sentence? Here are some example sentences to help you improve your vocabulary:
The levels of Ptc were relatively low in these lines and a standard immunoprecipitation procedure followed by western blotting was required to detect the Ptc-GFP protein.
Thus, in considering models of Ptc function based on Hh-stimulated effects it is important to consider whether the form of Smo that is being observed is the active (coupler/effector bound) signaling state, or perhaps a downregulated and inactive form.
Figure 5ashows an immunoprecipitation (anti-GFP) plus protein blot (anti-Ptc) analysis of extracts from these cells treated for 4, 8 and 24 hours with vehicle, 25 nM Hh protein or 0.2 μM Hh agonist (see Figure 1e; Hh-Ag 1.2).
We detected expression of the Hedgehog receptors Patched (Ptc) and Smoothened (Smo) at all stages (Figure 3B), consistent with the possibility that NS-derived cells are competent for Hh signaling.
Samples underwent 30 cycles of amplification with Perkin-Elmer Taq polymerase in a PTC Thermal Cycler (MJ Research, MA) (94° for 40 sec, 57° for 1 min, 72° for 2 min).
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