Words similar to proteolipid
Example sentences for: proteolipid
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Since etiolation results in rapid elongation of seedlings it was expected that there would be a concomitant increase in subunit A messenger RNA as seen with proteolipid subunit mRNA in A. thaliana [ 36 ] and subunit A message levels in rapidly expanding cotton ( Gossypium hirsutum ) fibers [ 73 ] . Equivalent loading of whole RNA was evaluated via spectrophotometry measuring absorption at 260 nm using a Perkin-Elmer Lambda-3 spectrophotometer and by visual confirmation using non denaturing ethidium bromide stained agarose gel electrophoresis (results not shown).
Three proteolipid genes were evaluated for their response to salt stress as well as etiolation [ 36 ] . All three genes exhibited an approximately two fold increase in mRNA levels in response to salt stress (50 mM).
The catalytic subunit exists as two distinct genes with highly conserved exons and intron boundaries in twelve species of plants [ 25 ] . In the flowering plant Daucus carota (carrot) evidence exists for two distinct isoforms for the catalytic subunit, one of which is tonoplast specific while the other is localized to the Golgi [ 26 ] . In addition, two distinct mRNAs have been isolated from cotton ( Gossypium hirsutum ) [ 27 ] . Two subunit A mRNAs are expressed in the unicellular alga Acetabularia acetabulum [ 28 29 ] . Isoforms of other V-ATPase subunits are also known from plants including subunit B [ 27 30 32 ] , the proteolipid subunit [ 33 34 35 36 37 ] and subunits D and E [ 38 ] .
Fully expanded leaves exhibited an increase only for the proteolipid mRNA, an increase of approximately three fold over non salt stressed plants [ 54 ] . Cell suspension cultures when subjected to salt stress exhibited increase in activity of both P-Type and V-Type ATPases without concomitant increase in protein levels [ 55 ] . Transcript levels of subunit E of the V-ATPase in Mesembryanthemum crystallinum were recently shown to increase in leaves but not roots of salt stressed plants with a concomitant increase in protein levels in leaves [ 56 ] . On the other hand, subunit E in Hordeum vulgare showed no effect on mRNA level in response to salt stress [ 57 ] . The effects of salt stress on V-ATPases has also been investigated in tobacco ( Nicotiana tabacum ) suspension cells.
Thus, some of us recently showed that administration of two self peptides that can induce EAE, myelin proteolipid protein peptide 139 to 151 (PLP139-151) or myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55), can result in severe anaphylactic reactions [ 14 ] . This result clearly indicated that severe allergic reactions to self peptides can occur in mice that have been induced to express pathology (i.e.
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