Words similar to prb
Example sentences for: prb
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These intriguing results indicate that the interaction of hypophosphorylated pRb2/p130 with E2F-4 followed by enhancement in nuclear location after SAHA treatment is a novel mechanism in SAHA-mediating cell growth arrest.
Protein (250 μg) from the control-infected or HIV-1-infected podocytes on day 14 of the differentiation protocol was also analyzed by western blot for total pRb (ECL detection of a 1:1000 dilution of rabbit anti-Rb, Santa Cruz Biotechnology) and phospho-Rb (Ser780) (ECL detection of a 1:500 dilution of rabbit anti-phospho-Rb (Ser780) antibody binding, Cell Signaling).
SAHA also resulted in the inhibition of G1/S kinase activities and, consequently, hypophosphorylation of the three pRb pocket proteins, which led to G1 cell growth arrest and dramatic decreases in DNA synthesis in both cell lines.
This protein behaves similarly if not identically to endogenous PP1α with respect to nuclear localization, complex formation to a recently described PP1-associated nuclear targeting protein, and in vitro enzymatic activity towards phosphorylase a and the cell growth and tumor suppressor protein pRB.
To assay pRB-directed phosphatase activity, [ 32P]-labeled pRB substrate was prepared by immunoprecipitation with monoclonal antibody to pRB, PMG3-245 (PharMingen), from [ 32P]-labeled CV-1P cells as described previously [ 20 ] . For the source of enzyme, immunocomplexed 6His-HA-PP1α from induced LLWO2F cells were mixed and incubated with [ 32P]-labeled immunoprecipitated pRB for 30 minutes at 30°C as previously described [ 20 ] . Phosphatase reactions were terminated by the addition of SDS-PAGE sample buffer and boiling.
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