Words similar to prb
Example sentences for: prb
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Protein (250 μg) from the control-infected or HIV-1-infected podocytes on day 14 of the differentiation protocol was also analyzed by western blot for total pRb (ECL detection of a 1:1000 dilution of rabbit anti-Rb, Santa Cruz Biotechnology) and phospho-Rb (Ser780) (ECL detection of a 1:500 dilution of rabbit anti-phospho-Rb (Ser780) antibody binding, Cell Signaling).
The difference in the level of hypophosphorylated pRb pocket protein isoforms between the two cell lines treated with SAHA may be attributed to differences in G1 and S phase cdk activity (examined in the following section).
SAHA also resulted in the inhibition of G1/S kinase activities and, consequently, hypophosphorylation of the three pRb pocket proteins, which led to G1 cell growth arrest and dramatic decreases in DNA synthesis in both cell lines.
5) and its nuclear localization after SAHA treatment was associated with interaction to E2F-4, equal amounts of nuclear protein extracts were immunoprecipitated with antibodies against E2F-4 followed by western blot analysis against pRb2/p130 antibodies.
Figure 6shows a significant decrease in the 32P radiolabel intensity for pRB following incubation with the anti-hemagglutinin immunoprecipitate compared to the normal mouse IgG control immunoprecipitate.