Words similar to prs
Example sentences for: prs
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To determine whether a multicopy lysyl tRNA synthetase plasmid affected accumulation of dinucleoside polyphosphates, we transformed HNT2 ADE2 strain BY71-16d and hnt2ΔADE2 strain BY71-6c with control plasmid pRS423 and with plasmid pM1.
For the models that account for correlation, however, scores from genomic DNA are higher on average than those computed for the PRS (Table 4, Figure 4).
All those vectors were created with the Bluescript-based yeast- E. coli shuttle vector pRS313 (HIS3-CEN6-ARSH4) [ 32 ] . The cassette containing the URA3 gene driven by the Shizosaccharomyces pombe ADH1 promoter was derived from the pBL94 plasmid [ 19 ] . In the original cassette, the ADH1 promoter and URA3 were separated by a 35-bp adaptor containing an out-of-frame ATG initiation codon.
Two independent PCR trials were completed for each mutant allele and cloned into the pRS315 vector [ 66 ] . Clones from each were obtained and both strands sequenced.
Then, a fragment containing the PPT1 ORF was excised from YCpIF16-PPT1 by Xho I and Not I digestion and subcloned into this plasmid to form pRS306-955HAPPT1.
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