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Example sentences for: prs

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Strain BY71-6c was transformed with plasmids pRS423 and pM1 (multicopy KRS1 ) and cultures were grown as for measurement of dinucleoside polyphosphate levels.
To confirm that these genomic fragments indeed carried the TAB genes, complementing fragments were cloned into the integrating vector [pRS305, LEU2], and linearized to target integration into the tab locus in a cdc15Δ::TRP1 tab [pMET3-CDC15, URA3 ] strain.
To construct [ pMET3-CDC15, URA3 ] (pWS100) and [ pMET3-cdc15-2, URA3 ] (pWS109), the corresponding genes (from start codon to 300 bps downstream of the stop codon) were amplified by PCR from genomic DNA of wild type (RJD381) or cdc15-2 (RJD619) cells, and cloned into the SpeI and SacII sites of RDB620 (a derivative of pRS316 containing the MET3 promoter inserted between the HindIII and EcoRI sites).
ADE2 HNT2 ) and BY71-6c ( ADE2 hnt2 ) were transformed with pM1 and the pRS423 control plasmid, and cultures were harvested at 24, 48 and 72 hours.
At 24, 48 and 72 hours of growth, 50 ml of cells were harvested, cells counted microscopically, lysed, and levels of AppppN and ApppN were determined as described [ 13 ] . Intracellular concentrations of ApppN and AppppN were calculated using 7 × 10 -14l as the volume of a haploid cell [ 50 ] . Cultures of BY71-6c were transformed with plasmids pRS423, pB05, pB32 and pB86 and transformants were selected on SDC-his media.