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Example sentences for: prs

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Then, a fragment containing the PPT1 ORF was excised from YCpIF16-PPT1 by Xho I and Not I digestion and subcloned into this plasmid to form pRS306-955HAPPT1.
Since both genomic fragments contained multiple open reading frames (ORFs), each ORF was amplified by PCR from yeast genomic DNA and cloned into the integrating plasmid pRS305.
This product, cloned into the Sma I restriction site of plasmid pRS423, generated plasmid pM1 in which KRS1 is oriented anti to HIS3 . Plasmids are summarized in Table 3.
The PCR fragment was digested with Sad and cloned into the SacI-SmaI sites of pRS426 to generate pHZ52.
At 24, 48 and 72 hours of growth, 50 ml of cells were harvested, cells counted microscopically, lysed, and levels of AppppN and ApppN were determined as described [ 13 ] . Intracellular concentrations of ApppN and AppppN were calculated using 7 × 10 -14l as the volume of a haploid cell [ 50 ] . Cultures of BY71-6c were transformed with plasmids pRS423, pB05, pB32 and pB86 and transformants were selected on SDC-his media.