Words similar to prs

Example sentences for: prs

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URA3 was amplified as a ~ 1.1 kb fragment from pRS306 [ 32 ] . Before TAR cloning, the vector was linearized with Bam HI to expose the B1 targeting sequence and the URA3 sequence placed at the end of the transgene-specific hook.
PCR reactions were carried out using as templates pRS vectors carrying the desired markers [ 25, 28].
At 24, 48 and 72 hours of growth, 50 ml of cells were harvested, cells counted microscopically, lysed, and levels of AppppN and ApppN were determined as described [ 13 ] . Intracellular concentrations of ApppN and AppppN were calculated using 7 × 10 -14l as the volume of a haploid cell [ 50 ] . Cultures of BY71-6c were transformed with plasmids pRS423, pB05, pB32 and pB86 and transformants were selected on SDC-his media.
All cdc15Δ tab mutants were also crossed to strains harboring either [pMET3-CDC15, URA3] [pRS313, HIS3] or [pMET3-CDC15, URA3] [pRS315, LEU2] to introduce these plasmids.
ADE2 HNT2 ) and BY71-6c ( ADE2 hnt2 ) were transformed with pM1 and the pRS423 control plasmid, and cultures were harvested at 24, 48 and 72 hours.