Words similar to prs

Example sentences for: prs

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URA3 was amplified as a ~ 1.1 kb fragment from pRS306 [ 32 ] . Before TAR cloning, the vector was linearized with Bam HI to expose the B1 targeting sequence and the URA3 sequence placed at the end of the transgene-specific hook.
The 1.8 kb SacI-SalI fragment of the original library clone, containing only the 5' half of NrG1 without the zinc fingers, was cloned into the SacI-SalI sites of pRS426 to create pHZ56.
This product, cloned into the Sma I restriction site of plasmid pRS423, generated plasmid pM1 in which KRS1 is oriented anti to HIS3 . Plasmids are summarized in Table 3.
The PCR fragments were inserted into a pRS313 polylinker [ 32 ] to generate the basic ARS -containing TAR vector designated as pARS-VN.
Each strain was transformed with a plasmid harboring the respective wild type gene in pRS316 (pSW1268 or pSW1269; see below) [ 66 ] and sporulated to isolate haploid secΔ deletion strains with the p SEC/URA3/CEN plasmid.