Words similar to prsvl
Example sentences for: prsvl
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The readthrough level was expressed as the ratio of standardized activity from the test construct to the standardized activity from the wild type pRSVL construct.
In the pRSVL74 derived plasmids, the luc gene encoding the very sensitive luciferase reporter is under the control of the strong Rous Sarcoma Virus promoter [ 24].
To evaluate readthrough efficiency, the luciferase activity obtained from a test construct was compared to the activity of the wild type pRSVL vector, which was used as an internal control of each individual transfection experiment.
As shown in Table 1, the activities of the modified luciferase proteins vary from 10% to 40% compared to the wild type pRSVL reporter, emphasizing the importance of this type of control.
The pRSVL74 cloning vector is shown in Figure 1and the procedures used to construct the mutant derivatives have been described previously [ 25].