Words similar to pr-tcm
Example sentences for: pr-tcm
How can you use “pr-tcm” in a sentence? Here are some example sentences to help you improve your vocabulary:
However, this by itself does not account fully for the anomalous mobility of pR on SDS-PAGE gels, because pR-TCM is still approximately 5,000 higher in molecular weight than bR according to SDS-PAGE (fig.
Therefore we compared the SDS-PAGE gel mobility of wild-type pR to that of a cys-less mutant (pR-TCM).
Subsequent SDS-PAGE analysis of pR samples that had been stored for periods of time up to several months indicate that after sitting for several weeks in octylglucoside solution at 4°C, the largest post-translational modification on wild-type pR is eliminated - presumably hydrolysed off of the cysteine(s) - leaving only a 31,000-MW band indistinguishable from that seen for pR-TCM (data not shown).
The elimination of the three possible sulfhydryl attachment sites lowers the apparent molecular weight of the pR-TCM by 5,000 (Fig.
The triple cysteine mutant (pR-TCM) has all three of its native cysteines (Cys-107, Cys-156, and Cys-175) replaced with serines, and was prepared using the same E. coli expression system and purification methods as the wild type.