Words similar to pr-tcm

Example sentences for: pr-tcm

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Therefore we compared the SDS-PAGE gel mobility of wild-type pR to that of a cys-less mutant (pR-TCM).
pR, proteorhodopsin; bR, bacteriorhodopsin; OG, β-octyl-D-glucoside; SDS, sodium dodecylsulfate; PAGE, polyacrylamide gel electrophoresis; pR-TCM, pR triple cysteine mutant [C(107,156,175)S]; DHPC, 1,2-diheptanoyl-SN-glycero-3-phosphocholine; DMPC, dimyristoylphosphatidylcholine; HEPES, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
The triple cysteine mutant (pR-TCM) has all three of its native cysteines (Cys-107, Cys-156, and Cys-175) replaced with serines, and was prepared using the same E. coli expression system and purification methods as the wild type.
The elimination of the three possible sulfhydryl attachment sites lowers the apparent molecular weight of the pR-TCM by 5,000 (Fig.
This doublet is also present in the less-purified sample of pR-TCM, with both bands shifted down by approximately the same amount (fig.