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Example sentences for: ppacpl

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To determine which domains in DFz2 are important for its synergistic activity with Wg in the S2 cell assay, we deleted the regions encoding the CRD domain (DFz2ΔCRD) or the cytoplasmic tail (DFz2ΔC) in pPacPL (Figure 3A) and tested the deletion constructs shown in panel A in the luciferase assay described earlier (Figure 3B).
The final amount of DNA was adjusted to an equal level using pPacPL vector DNA.
The activation of Wnt signaling by various effectors in pPacPL was then determined by measurement of relative luciferase activity (Figure 1B).
All Drosophila expression constructs were cloned in the vector pPacPL [ 27 28 ] . Cells grown in 6-well plates were transfected with 0.5 μg of indicated plasmid together with the reporter plasmids using Cellfectin reagent (Invitrogen Life Technologies).
To investigate whether these constructs can also function in S2 cells and whether their activities can synergize with Wg, we transferred LRP6 and LRP6ΔN into the Drosophila expression vector pPacPL (Figure 4A) and tested them alone and with various combinations of Wg and DFz2 in S2 cells (Figure 4B).