Words similar to phosphorylase
- phosphorimage
- phosphorimager
- phosphorlmager
- phosphorodithioate
- phosphorothioate
- phosphorous
- phosphorplate
- phosphorus
- phosphoryation
- phosphoryl
- phosphorylase
- phosphorylases
- phosphorylatable
- phosphorylate
- phosphorylated
- phosphorylates
- phosphorylation
- phosphorylation-activation
- phosphotase
- phosporimager
Example sentences for: phosphorylase
How can you use “phosphorylase” in a sentence? Here are some example sentences to help you improve your vocabulary:
Protein kinase A (3':5'-cyclic AMP dependent), phosphorylase b (EC 2.4.
[ 38 ] Briefly, radiolabeled phosphorylase a was synthesized by taking phosphorylase b (Sigma), at a concentration of 10 mg/ml, and incubating with 0.2 mg/ml phosphorylase kinase (Sigma) for 1.5 hours at 30°C in kinase reaction buffer (100 mM Tris-HCl; 100 mM sodium glycerol-1-phosphate, pH 8.2; 0.1 mM CaCl 2 , 10 mM magnesium acetate, and 0.2 mM [g- 32P]ATP {10 6cpm/nmol}).
This protein behaves similarly if not identically to endogenous PP1α with respect to nuclear localization, complex formation to a recently described PP1-associated nuclear targeting protein, and in vitro enzymatic activity towards phosphorylase a and the cell growth and tumor suppressor protein pRB.
To inhibit any endogenous PP2A activity, which can also dephosphorylate phosphorylase a and thus interfere with PP1 activity measurements, these assays were carried out in the presence of 5 nM okadaic acid, which does not inhibit PP1 activity [ 30 ] . Although reproducible, we observed only modest increases in PP1-specific activity found in lysates from induced cells compared to the uninduced controls (Figure 7).
Phosphorylase phosphatase activity was measured as the release of trichloroacetic acid (TCA) soluble counts from 32P-phosphorylase a according to Cohen et.
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