Words similar to phosphorylase

Example sentences for: phosphorylase

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Protein kinase A (3':5'-cyclic AMP dependent), phosphorylase b (EC 2.4.
[ 38 ] Briefly, radiolabeled phosphorylase a was synthesized by taking phosphorylase b (Sigma), at a concentration of 10 mg/ml, and incubating with 0.2 mg/ml phosphorylase kinase (Sigma) for 1.5 hours at 30°C in kinase reaction buffer (100 mM Tris-HCl; 100 mM sodium glycerol-1-phosphate, pH 8.2; 0.1 mM CaCl 2 , 10 mM magnesium acetate, and 0.2 mM [g- 32P]ATP {10 6cpm/nmol}).
Our results support the notion that induced PP1α protein functions identically to endogenous PP1α with respect to non-catalytic subunit binding and activity towards phosphorylase a and the tumor suppressor pRB.
To inhibit any endogenous PP2A activity, which can also dephosphorylate phosphorylase a and thus interfere with PP1 activity measurements, these assays were carried out in the presence of 5 nM okadaic acid, which does not inhibit PP1 activity [ 30 ] . Although reproducible, we observed only modest increases in PP1-specific activity found in lysates from induced cells compared to the uninduced controls (Figure 7).
M r 21,500), carbonic anhydrase ( M r 31,000), ovalbumin ( M r 45,000), bovine serum albumin ( M r 66,000) and phosphorylase b ( M r 97,400) were used as molecular mass standards.