Words similar to phenylmethylsulfonyl
- phenotypic
- phenotypically
- phenotyping
- phentermine
- phenylacetaldehyde
- phenylglycine
- phenylindole
- phenylketonuria
- phenylmethyl
- phenylmethyl-sulfonyl
- phenylmethylsulfonyl
- phenylmethylsulfonylfluoride
- phenylmethylsulphonyl
- phenylphenalenone
- phenylpiperidine
- phenylpropanoid
- phenylsepharose
- phenytoin
- pheonyx
- pheriche
Example sentences for: phenylmethylsulfonyl
How can you use “phenylmethylsulfonyl” in a sentence? Here are some example sentences to help you improve your vocabulary:
Control and treated cells were lysed by repeated freeze-thaw cycles with buffer containing 10 mM HEPES (pH 7.5), 1.5 mM Mg(OAc) 2 , 1 mM dithiothreitol (DTT), 0.5% NP40, 5% glycerol, 0.5 mM phenylmethylsulfonyl fluoride, and 10 μg/ml each of the protease inhibitors aprotinin, pepstatin, and leupeptin.
Protein in positive elution fractions was refolded to native structure by serial dialysis against buffer D (20 mm HEPES, 0.1 M KCl, 0.2 mm EDTA, 0.5 mm dithiothreitol, 0.5 mm phenylmethylsulfonyl fluoride, 20% glycerol) containing stepwise decreasing concentrations of urea (6 M for 2 hours, 4 M for 2 hours, 2 M for 2 hours, no urea for 12 hours).
aa, amino acid; Ab, antibody; bp, base pair; CaPO 4 , calcium phosphate; C/EBPβ, CCAAT-enhancer binding protein-beta; CAT, chloramphenicol acetyl transferase; DMEM, Dulbecco's Modified Eagle Medium; GST, glutathione-S-transferase; MAPK, mitogen activated protein kinase; PBS, phosphate buffered saline; PMSF, phenylmethylsulfonyl fluoride; SRE, serum response element; SRF, serum response factor; TCF, ternary complex factor.
For preparation of protein lysates from tissue samples (levator ani muscle; rectus fascia = ~negative control; and whole placenta = low p27 positive control) 100 tissue sections (7 μm thick) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5,200 mM NaCI, 0.25% Nonidet P-40) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride (400 μl/100 mg of tissue sections).
15 (50 mM HEPES/KOH, pH 7.5, 150 mM KCl, 0.02% Nonidet P-40, 5 mM magnesium acetate, 1 mM EDTA, 1 mM EGTA, 10% glycerol, 1 mM phenylmethylsulfonyl fluoride, 2 μg/ml pepstatin, 2 μg/ml leupeptin, 5 μg/ml aprotinin, 1 mM dithiothreitol).
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