Words similar to phenylmethylsulfonyl
- phenotypic
- phenotypically
- phenotyping
- phentermine
- phenylacetaldehyde
- phenylglycine
- phenylindole
- phenylketonuria
- phenylmethyl
- phenylmethyl-sulfonyl
- phenylmethylsulfonyl
- phenylmethylsulfonylfluoride
- phenylmethylsulphonyl
- phenylphenalenone
- phenylpiperidine
- phenylpropanoid
- phenylsepharose
- phenytoin
- pheonyx
- pheriche
Example sentences for: phenylmethylsulfonyl
How can you use “phenylmethylsulfonyl” in a sentence? Here are some example sentences to help you improve your vocabulary:
Before electrophoresis, slices were incubated twice for 30 min in 1.0 mM phenylmethylsulfonyl fluoride (PMSF) (Sigma.
Protein in positive elution fractions was refolded to native structure by serial dialysis against buffer D (20 mm HEPES, 0.1 M KCl, 0.2 mm EDTA, 0.5 mm dithiothreitol, 0.5 mm phenylmethylsulfonyl fluoride, 20% glycerol) containing stepwise decreasing concentrations of urea (6 M for 2 hours, 4 M for 2 hours, 2 M for 2 hours, no urea for 12 hours).
Control and treated cells were rinsed twice with PBS and then incubated with 1 ml of lysis buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl 2 , 50 mM NaF, 5 mM sodium pyrophosphate, 0.2 mM sodium orthovanadate, 2 μg/ml aprotinin, 2 μg/ml leupeptin, 10% glycerol, 1% Triton X-100, 0.5% NP-40, 1 mM phenylmethylsulfonyl fluoride) for 1 hour with shaking at 4°C.
The cell pellet was washed once in cold phosphate-buffered saline and subsequently resuspended in hypotonic lysis buffer (10 mM Tris.Cl, pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 1 mM phenylmethylsulfonyl fluoride, 2 μg/ml pepstatin, 2 μg/ml leupeptin, 5 μg/ml aprotinin, 1 mM dithiothreitol).
Thirty μl of phenylmethylsulfonyl fluoride (PMSF), 10 mg/ml, was added per gram of tissue and incubated on ice for 30 min.
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