Words similar to phenylmethylsulfonyl

Example sentences for: phenylmethylsulfonyl

How can you use “phenylmethylsulfonyl” in a sentence? Here are some example sentences to help you improve your vocabulary:

Nuclear extracts were obtained as described elsewhere [ 19 ] . Briefly, detached cells were resuspended in buffer A (10 mM Hepes pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM dithiothreitol, 0.5 mM phenylmethylsulfonyl fluoride) and cells were lysed with Nonidet P-40.
Control and treated cells were lysed by repeated freeze-thaw cycles with buffer containing 10 mM HEPES (pH 7.5), 1.5 mM Mg(OAc) 2 , 1 mM dithiothreitol (DTT), 0.5% NP40, 5% glycerol, 0.5 mM phenylmethylsulfonyl fluoride, and 10 μg/ml each of the protease inhibitors aprotinin, pepstatin, and leupeptin.
Control and treated cells were rinsed twice with PBS and then incubated with 1 ml of lysis buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl 2 , 50 mM NaF, 5 mM sodium pyrophosphate, 0.2 mM sodium orthovanadate, 2 μg/ml aprotinin, 2 μg/ml leupeptin, 10% glycerol, 1% Triton X-100, 0.5% NP-40, 1 mM phenylmethylsulfonyl fluoride) for 1 hour with shaking at 4°C.
Cells were harvested by centrifugation and washed once with phosphate-buffered saline (PBS) containing the following protease inhibitors: 2 mg/ml aprotinin, 2 mg/ml leupeptin, 2 mM phenylmethylsulfonyl fluoride (PMSF), and 0.2 mM pepstatin.
2 mM phenylmethylsulfonyl fluoride and protease inhibitors and sonicated.