Words similar to phenylsepharose

Example sentences for: phenylsepharose

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Time-resolved UV/vis spectroscopy methods were as described previously [ 18 ] . A Phenylsepharose™-purified pR sample was reconstituted into mixed micelles containing 1,2-diheptanoyl-SN-glycero-3-phosphocholine (DHPC), by adding a 1% solution of the short-chain lipid and then removing most of the detergent on a Sephadex G-25 column equilibrated with 1% DHPC in 100 mM NaCl.
The Phenylsepharose column increases the purity level to 24%, with approximately 5% loss.
A portion of the Phenylsepharose™-purified pR (1.
Phenylsepharose™ column chromatography separates proteins on the basis of hydrophobicity, and has been used previously in hR purification [ 8 9 10 ] . As an initial purification step, the Phenylsepharose™ column achieved a substantial increase in protein purity to 25%, along with removal of most of the lipid.
The diluted OG-solubilized membrane extract (10 mg in 300 mL total volume of 0.5% OG) was loaded on a 25 × 1 cm column containing Phenylsepharose™ (6 fast flow high sub; Amersham Pharmacia Biotech).