Example sentences for: nuclease

How can you use “nuclease” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After removal of the nuclease, the plasmids were linearized with HindIII.

  • The recD mutant does not show nuclease activity but is recombination-proficient and able to repair the broken DNA molecules [ 6 ] . This explains why it does not accumulate the huge linear forms.

  • First, Dlakic [ 12 ] and Hofmann et al [ 13 ] showed that the yCCR4 C-terminus contains a fold related to the Mg 2+-dependent endonuclease core, suggesting that it may function as a nuclease.

  • Bacterial RNase III orthologues exhibit the simplest primary structure, which includes a C-terminal dsRNA-binding motif (dsRBM), and an N-terminal catalytic (nuclease) domain (Figure 1).

  • The cell lysate was clarified at 13,000×g for 5 min and digested with 100 units micrococcal nuclease (Roche) for 45 min at 37°C in the presence of 10 mM CaCl 2 . Digestion was stopped by addition of EDTA to 15 mM, and then the lysate was clarified again by centrifugation at 13,000×g for 5 min.


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