Example sentences for: nuclease-free

How can you use “nuclease-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Full-length ds-cDNA was synthesized by adding 57 μl nuclease-free water, 10 μl 10× PCR buffer (Roche), 10 μl 25 mM MgCl 2 , 1 μl 10 mM dNTP and 5 u Ampli-Taq Gold DNA Polymerase (Roche).

  • 4 μL nuclease-free water.

  • The pellet was dried at 22-25°C for 10 min and re-suspended in 50 μl of nuclease-free H 2 O. The re-suspended solution was heated at 56°C for 5 min and centrifuged at 10,000 rpm for 2 min.

  • Ninety-six plasmids for each tester were randomly selected and plasmid DNA was extracted (Wizard SV96 Plasmid DNA Purification System, Promega Corporation, Madison, WI) and eluted into 100 μl sterile nuclease-free water.

  • The RNA sample was added to a sterile RNase-free micro-centrifuge tube, and nuclease-free water was added to 9.5 μl.


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