Words similar to nuclease-resistant
Example sentences for: nuclease-resistant
How can you use “nuclease-resistant” in a sentence? Here are some example sentences to help you improve your vocabulary:
The RNA genome of RSV is about 15 kb long and contains 11 documented protein-coding genes [ 13 ] . Three viral proteins are minimally required to reconstitute the functional transcription complex of NNR viruses [ 3 ] : the nucleocapsid protein (N) that wraps the negative-strand genome RNA and its full-length complement, the positive-strand antigenome RNA, thus converting them into highly nuclease-resistant, chromatin-like templates; the large protein (L), which is the major subunit of the RdRP; and the phosphoprotein (P), which is the smaller subunit of RdRP and an essential transcription factor of L [ 19 20 21 22 ] . In RSV, optimal transcription, although not replication, additionally requires the transcription antitermination protein M2-1 [ 13 ] . In addition, cellular actin, and to a lesser extent, profilin, are also required for viral transcription [ 17 18 ] .
As mentioned before, the N-encapsidated genome-length RNAs are indeed extremely resistant to nucleases, to the extent that formation of such nuclease-resistant RNA products is in fact considered a defining criterion for viral replication in vitro and in vivo [ 32 ] . While the resistance of the viral genomic RNA to dsRNA has important ramifications for antiviral therapy, it is also clear that the dsRNA approach cannot be used against cis -acting NNR viral genomic sequences such as the intergenic regions, for which the cDNA-based approach will continue to be the method of choice [ 16 ] . It is obvious that a creative combination of the two techniques will lead to exciting possibilities in the reverse genetics of RNA viruses and in the antiviral regimen.
Loading...