Words similar to npa
Example sentences for: npa
How can you use “npa” in a sentence? Here are some example sentences to help you improve your vocabulary:
In HsAQP1, the cysteine residue that confers sensitivity is C189, adjacent to the second NPA motif, but no plant AQP shows a cysteine in this position although effects of mercury have been documented in plants in many studies.
A genomic fragment containing BET3 complemented the ts phenotype of npa10 , and npa3-1 was rescued by a library plasmid only when it contained full length SEC27 (see Materials and Methods).
Overall, the large number of independent npa mutants (potentially 87) was surprising and suggested that a number of distinct proteins can affect NPC assembly and localization.
Both regions contained multiple open reading frames; however, we focused immediately on a single gene for each mutant, SEC13 for npa2-1 and SEC23 for npa1-1 . SEC13 was first identified in a screen for secretory mutants and encodes a protein of the COPII coat, which is required for vesicle budding from the ER [ 37 38 39 ] . More recently, it has been shown that a fraction of the total cellular Sec13p co-fractionates with NPCs in a distinct subcomplex with Nup84p, Nup85p, Nup120p, Nup145Cp and Seh1p [ 40 41 ] . SEC23 was also identified in a screen for secretory mutants, and it, too, is a component of the COPII coat [ 37 38 39 ] . Co-purification of Sec23p with intact NPCs has not been reported [ 5 ] ; however, Sec23p was recently isolated in Nup42p and Nup116p affinity chromatography strategies [ 42 ] . Expression constructs containing only SEC13 or SEC23 were transformed into the mutant strains and assayed for their ability to rescue growth at the non-permissive temperature.
Our continued efforts focused on cloning additional npa mutants by library complementation.
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