Example sentences for: npcs
How can you use “npcs” in a sentence? Here are some example sentences to help you improve your vocabulary:
NPC clusters, NE herniations, or membrane sealing of NPCs) [ 23 31 35 ] , and in previous studies GFP-epitope tagging of Nic96p or Nup170p did not inhibit function [ [ 26 ] , our unpublished observations].
Mutants perturbing GFP-Nup localization could include cells with 1) NPC clusters in NE subregions, 2) mislocalization of the GFP-Nup or aggregated NPC subcomplexes to the cytoplasm or nucleus (due to either a block in new assembly or increased instability of existing NPCs), 3) mislocalization of the GFP-Nup due to indirect perturbation of the ER and NE membrane, or 4) decreased GFP-Nup incorporation followed by turnover resulting in cells with diminished total fluorescence.
In fact, Hurt and coworkers have recently demonstrated the in vitro assembly of a Nup subcomplex from entirely recombinant proteins [ 20 ] . Given the structural and functional similarities between vertebrate and yeast NPCs and the insertion of both into a pre-existing NEs, it is likely that NPC biogenesis occurs via a similar mechanism in all organisms.
Both regions contained multiple open reading frames; however, we focused immediately on a single gene for each mutant, SEC13 for npa2-1 and SEC23 for npa1-1 . SEC13 was first identified in a screen for secretory mutants and encodes a protein of the COPII coat, which is required for vesicle budding from the ER [ 37 38 39 ] . More recently, it has been shown that a fraction of the total cellular Sec13p co-fractionates with NPCs in a distinct subcomplex with Nup84p, Nup85p, Nup120p, Nup145Cp and Seh1p [ 40 41 ] . SEC23 was also identified in a screen for secretory mutants, and it, too, is a component of the COPII coat [ 37 38 39 ] . Co-purification of Sec23p with intact NPCs has not been reported [ 5 ] ; however, Sec23p was recently isolated in Nup42p and Nup116p affinity chromatography strategies [ 42 ] . Expression constructs containing only SEC13 or SEC23 were transformed into the mutant strains and assayed for their ability to rescue growth at the non-permissive temperature.
Electron dense structures resembling NPCs were also embedded in membranes distinct from the nucleus/NE (Figure 4F).
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