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Example sentences for: npcs

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In fact, Hurt and coworkers have recently demonstrated the in vitro assembly of a Nup subcomplex from entirely recombinant proteins [ 20 ] . Given the structural and functional similarities between vertebrate and yeast NPCs and the insertion of both into a pre-existing NEs, it is likely that NPC biogenesis occurs via a similar mechanism in all organisms.
We did not observe any evidence of cytoplasmic annulate lamellae formation, although nuclear invaginations sometimes resulted in juxtaposed double membranes containing NPCs (Figure 4F).
After staining with osmium tetroxide and uranyl acetate, NPCs in wild-type/parental cells were represented by electron dense structures spanning the pore (Figure 4).
Electron dense structures resembling NPCs were also embedded in membranes distinct from the nucleus/NE (Figure 4F).
This strategy was based on the ability to functionally express GFP-nucleoporin fusions, and the assumption that NPC a ssembly/mislocalization mutants expressing a GFP-nucleoporin would have distinct fluorescence properties [ 26 ] . In wild type yeast cells, fluorescence microscopy localization of proteins at NPCs appears as punctate staining of virtually the entire nuclear rim circumference (see Figure 2).