Example sentences for: npcs
How can you use “npcs” in a sentence? Here are some example sentences to help you improve your vocabulary:
Using GFP-tagged NPCs, we designed live cell assays to monitor NPC dynamics and assembly rates [ 25 ] . Moreover, to isolate factors required for proper NPC assembly and localization, we developed a novel genetic screening strategy.
The three dimensional structure of NPCs from both Xenopus and budding yeast Saccharomyces cerevisiae is characterized by distinct substructures that are arranged with an 8-fold rotational symmetry [ 3 ] . Eight spoke-like structures form the central NPC core and are flanked on the cytoplasmic and nuclear sides by ring structures.
Both regions contained multiple open reading frames; however, we focused immediately on a single gene for each mutant, SEC13 for npa2-1 and SEC23 for npa1-1 . SEC13 was first identified in a screen for secretory mutants and encodes a protein of the COPII coat, which is required for vesicle budding from the ER [ 37 38 39 ] . More recently, it has been shown that a fraction of the total cellular Sec13p co-fractionates with NPCs in a distinct subcomplex with Nup84p, Nup85p, Nup120p, Nup145Cp and Seh1p [ 40 41 ] . SEC23 was also identified in a screen for secretory mutants, and it, too, is a component of the COPII coat [ 37 38 39 ] . Co-purification of Sec23p with intact NPCs has not been reported [ 5 ] ; however, Sec23p was recently isolated in Nup42p and Nup116p affinity chromatography strategies [ 42 ] . Expression constructs containing only SEC13 or SEC23 were transformed into the mutant strains and assayed for their ability to rescue growth at the non-permissive temperature.
In Figure 4E, a single cell cross-section showed multiple layers of NE containing NPCs.
Mutants perturbing GFP-Nup localization could include cells with 1) NPC clusters in NE subregions, 2) mislocalization of the GFP-Nup or aggregated NPC subcomplexes to the cytoplasm or nucleus (due to either a block in new assembly or increased instability of existing NPCs), 3) mislocalization of the GFP-Nup due to indirect perturbation of the ER and NE membrane, or 4) decreased GFP-Nup incorporation followed by turnover resulting in cells with diminished total fluorescence.
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