Example sentences for: npcs
How can you use “npcs” in a sentence? Here are some example sentences to help you improve your vocabulary:
Mutants perturbing GFP-Nup localization could include cells with 1) NPC clusters in NE subregions, 2) mislocalization of the GFP-Nup or aggregated NPC subcomplexes to the cytoplasm or nucleus (due to either a block in new assembly or increased instability of existing NPCs), 3) mislocalization of the GFP-Nup due to indirect perturbation of the ER and NE membrane, or 4) decreased GFP-Nup incorporation followed by turnover resulting in cells with diminished total fluorescence.
NPC clusters, NE herniations, or membrane sealing of NPCs) [ 23 31 35 ] , and in previous studies GFP-epitope tagging of Nic96p or Nup170p did not inhibit function [ [ 26 ] , our unpublished observations].
The global architectures of the yeast and vertebrate NPCs are highly similar, although there may be differences in the core ring structures [ 4 ] . Genetic and biochemical studies in yeast have identified ~30 NPC proteins (termed nucleoporins, Nups) that comprise the estimated ~44 MDa structure [ 5 ] . Recent proteomic analysis has determined that mammalian NPCs are also comprised of ~30 different proteins [ 6 ] ; however, the overall size of the vertebrate NPC is predicted to be larger [ 3 6 7 8 ] . Despite their size differences, yeast and vertebrate NPCs are responsible for identical transport reactions, and therefore, both structures are predicted to be functionally equivalent [ 9 ] .
Electron dense structures resembling NPCs were also embedded in membranes distinct from the nucleus/NE (Figure 4F).
Although it was not always possible to definitively identify a nucleus in each section (Figure 4F, for example), NPCs were present in the envelope juxtaposed to the nucleus despite the expanded membranes (Figure 4Cand 4D).
Loading...