Words similar to np-

Example sentences for: np-

How can you use “np-” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.6), 5 mM EDTA, 150 mM NaCl, 0.5 % NP-40, and 0.5% Triton X-100 containing 1 μg/ml leupeptin, aprotinin and antipain; 1 mM sodiumorthovanadate; and 0.5 mM PMSF (all reagents were obtained from Sigma Chemical Co.).
HA-NFATp was subsequently eluted with 1 mg/ml HA peptide resuspended in elution buffer (20 mM Tris (pH 7.9), 20% (v/v) glycerol, 1 mM EDTA, 5 mM MgCl 2 , 0.2 M NaCl, 0.1% NP-40, 0.2 mM PMSF, 1 μg/ml leupeptin, 1.4 μg/ml pepstatin A, and 1 mM DTT).
3], 75 mmol/l KCl, 3 mmol/l MgCl 2 , 1 mmol/l DTT,10 μmol/l dNTP [Sigma], 5 U/ml PRIME RNase Inhibitor [5 Prime ?? 3 Prime Incorporated, Boulder, CO, USA], 300 U/ml RNAguard [Pharmacia Biotech], 200 ng/ml oligo [dT] 24 [Integrated DNA Technologies Incorporated, Coralville, IA, USA], and 0.5% NP-40).
Nuclear extracts were prepared as previously described [ 14 ] . Briefly, cells were incubated in buffer (10 mM HEPES pH 7.4, 10 mM KCl, 2 mM MgCl 2 , 2 mM EDTA) on ice for 10 min followed by incubation with 10% NP-40.
The cell pellets were suspended in 150-300 μl ice-cold RIPA lysis buffer (1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS in PBS, 0.5 mM Phenylmethylsulfonyl fluoride (PMSF), 0.02 mg/ml Aprotinin, 1 mM Na 3 VO 4 ) and incubated for 30 min on ice.