Words similar to np-
Example sentences for: np-
How can you use “np-” in a sentence? Here are some example sentences to help you improve your vocabulary:
For example, the N-terminal domain of moesin lacking the C-terminal F-actin binding domain remained strongly associated with the NP-40-insoluble material of NIH3T3 cells [ 22].
Finally, blots were washed twice in TNE 50 plus 0.1% NP-40 and placed on a PhosphorImager cassette for further analysis.
Stimulated cells were then resuspended in Lysis Buffer containing 5% nonidet P-40 (NP-40, Sigma-Aldrich, Inc.).
Control and treated cells were rinsed twice with PBS and then incubated with 1 ml of lysis buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl 2 , 50 mM NaF, 5 mM sodium pyrophosphate, 0.2 mM sodium orthovanadate, 2 μg/ml aprotinin, 2 μg/ml leupeptin, 10% glycerol, 1% Triton X-100, 0.5% NP-40, 1 mM phenylmethylsulfonyl fluoride) for 1 hour with shaking at 4°C.
Cell were harvested by low-speed centrifugation and resuspended in lysis buffer (20 mM Tris (pH 7.9), 20% (v/v) glycerol, 1 mM EDTA, 0.5 M NaCl, 0.1% NP-40, 0.2 mM PMSF, 1 μg/ml leupeptin, 1.4 μg/ml pepstatin A, and 1 mM DTT).