Words similar to np-

Example sentences for: np-

How can you use “np-” in a sentence? Here are some example sentences to help you improve your vocabulary:

All cells were cultured to mid-log phase of growth, washed with PBS without Ca 2+and Mg 2+, and lysed in a buffer containing 50 mM Tris-HCl (pH 7.5), 120 mM NaCl, 5 mM EDTA, 50 mM NaF, 0.2 mM Na 3 VO 4 , 1 mM DTT, 0.5% NP-40 and protease inhibitors (Protease inhibitor cocktail tablets, Boehringer Mannheim, one tablet per 50 ml).
Nuclear extracts were prepared as previously described [ 14 ] . Briefly, cells were incubated in buffer (10 mM HEPES pH 7.4, 10 mM KCl, 2 mM MgCl 2 , 2 mM EDTA) on ice for 10 min followed by incubation with 10% NP-40.
The harvested cells (typically 5 × 10 6to 1 × 10 7cells) were resuspended in 300 μl of 8 μg/ml propidium iodide (Molecular Probes) in PBS containing 0.1% NP-40 and 10 μg/ml RNAse A, then incubated at room temperature in the dark for 30 minutes.
The cells were lysed with HMK-NP40 (50 mM Hepes, pH 7.5, 40 mM magnesium acetate, 20 mM potassium phosphate, 5% sucrose, 0.4% NP-40), clarified, and protein concentration determined as above.
Mouse tissues were homogenized in the RIPA lysis buffer (150 mM NaCl, 1.0% NP-40, 0.5% DOC, 0.1% SDS, 50 mM Tris, pH 8.0) at a concentration of 0.2 g tissue per ml of buffer.