Words similar to np-

Example sentences for: np-

How can you use “np-” in a sentence? Here are some example sentences to help you improve your vocabulary:

For example, the N-terminal domain of moesin lacking the C-terminal F-actin binding domain remained strongly associated with the NP-40-insoluble material of NIH3T3 cells [ 22].
Control and treated cells were rinsed twice with PBS and then incubated with 1 ml of lysis buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl 2 , 50 mM NaF, 5 mM sodium pyrophosphate, 0.2 mM sodium orthovanadate, 2 μg/ml aprotinin, 2 μg/ml leupeptin, 10% glycerol, 1% Triton X-100, 0.5% NP-40, 1 mM phenylmethylsulfonyl fluoride) for 1 hour with shaking at 4°C.
The harvested cells (typically 5 × 10 6to 1 × 10 7cells) were resuspended in 300 μl of 8 μg/ml propidium iodide (Molecular Probes) in PBS containing 0.1% NP-40 and 10 μg/ml RNAse A, then incubated at room temperature in the dark for 30 minutes.
Fourth, to control for possible inactivation of the RNAseH active site by low pH treatment, cores were permeabilized by treatment with 1 M NaCl or 0.3%SDS/1% NP-40 [ 16], again without yielding detectable RNAseH activity (data not shown).
All cells were cultured to mid-log phase of growth, washed with PBS without Ca 2+and Mg 2+, and lysed in a buffer containing 50 mM Tris-HCl (pH 7.5), 120 mM NaCl, 5 mM EDTA, 50 mM NaF, 0.2 mM Na 3 VO 4 , 1 mM DTT, 0.5% NP-40 and protease inhibitors (Protease inhibitor cocktail tablets, Boehringer Mannheim, one tablet per 50 ml).