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Example sentences for: np-

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For fluorescence-activated cell sorting (FACS) analysis, cells were stained with a cocktail of propidium iodide (PI) buffer (PBS with Ca2+ and Mg2+, RNase A [10 μg/ml], NP-40 [0.
Cells were lysed in a phosphate lysis buffer to prevent protein dephosphorylation (200 mM sodium phosphate, pH 7.5, 20 mM KCl, 5% sucrose, 0.5% NP-40, 1 mM PMSF).
The eggs were extracted in Celis lysis buffer (see [ 46 ] ) containing 2% (v:v) NP-40, 9.8 M urea, 100 mM dithiothreitol (DTT), 2% ampholines (pH 3.5-10), and protease inhibitors for 30 min at room temperature, according to the protocol described in [ 47 ] . Isoelectric focusing of the egg extracts (IEF) was performed using the Mini-PROTEAN II tube cell apparatus according to the manufacturer's protocol (Bio-Rad, Richmond, CA) with ampholines of pH 3.5-5 (30%), 3.5-10 (40%), 5-7 (20%), and 7-9 (10%), (Pharmacia Biotech, Uppsala, Sweden).
PBB was freshly prepared in 24 ml aliquots composed of 20 ml A-150 (20 mM Hepes, 150 mM KCl, 10 mM MgCl2 and 1% glycerol), and 2 ml each of phosphate buffered saline supplemented, respectively, with 1% Triton X-100 and 1% NP-40.
Cell pellets (10 7) were then resuspended directly in lysis buffer containing 150 mM NaCl, 1 mM MgCl 2 · 6H 2 O, 80 mM Tris-HCl and 0.1% NP-40.