Words similar to np-

Example sentences for: np-

How can you use “np-” in a sentence? Here are some example sentences to help you improve your vocabulary:

The cell lysate was mixed with 0.25 ml of Buffer B (Buffer A containing 1% NP-40 and 1% deoxycholate) and the insoluble material was removed by centrifugation at 100,000 × g for 30 min.
They were then digested (18 hr, at 37°C) with 0.5 units of recombinant N-glycanase in the presence of 2.5% NP-40, and subjected to SDS-PAGE and silver staining.
For example, the N-terminal domain of moesin lacking the C-terminal F-actin binding domain remained strongly associated with the NP-40-insoluble material of NIH3T3 cells [ 22].
Equal amounts (200 μg) of protein from each sample were immunoprecipitated with 0.5 μg cdk2 antibody in 300 μl IP buffer [50 mM Tris (pH 7.5), 5 mM EDTA, 20 mM glycerol, 150 mM NaCl, and 1% NP-40] and 25 μl protein A/G agarose beads (Santa Cruz Biotechnology) on an orbital shaker overnight at 4°C.
All cells were cultured to mid-log phase of growth, washed with PBS without Ca 2+and Mg 2+, and lysed in a buffer containing 50 mM Tris-HCl (pH 7.5), 120 mM NaCl, 5 mM EDTA, 50 mM NaF, 0.2 mM Na 3 VO 4 , 1 mM DTT, 0.5% NP-40 and protease inhibitors (Protease inhibitor cocktail tablets, Boehringer Mannheim, one tablet per 50 ml).