Words similar to np-

Example sentences for: np-

How can you use “np-” in a sentence? Here are some example sentences to help you improve your vocabulary:

The next day, blots were incubated with 10 ml of 125I-protein G (Amersham, 50 μl/10 ml solution) in TNE 50 plus 0.1% NP-40 for 2 hrs at 4°C.
0.5 mg of RNA were layered onto a 11 ml linear sucrose gradient (15-50% sucrose (w/v) in polysome buffer without NP-40), and the gradients were centrifuged in a Beckman SW40Ti rotor at 28000 rpm for 4 hours at 7°C.
Immunoprecipitates were collected by centrifugation (11000 × g for 10 min at 4°C), washed five times with 500 μl TG-buffer containing 20 mM Tris, 250 mM NaCl, and 0.5% NP-40.
The cell pellets were suspended in 150-300 μl ice-cold RIPA lysis buffer (1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS in PBS, 0.5 mM Phenylmethylsulfonyl fluoride (PMSF), 0.02 mg/ml Aprotinin, 1 mM Na 3 VO 4 ) and incubated for 30 min on ice.
Equal amounts (200 μg) of protein from each sample were immunoprecipitated with 0.5 μg Cdk2 antibody in 300 μl IP buffer (50 mM Tris, pH 7.5; 5 mM EDTA, 20 mM glycerol, 150 mM NaCl, 1% NP-40) and 25 μl protein A/G agarose beads (Santa Cruz Biotechnology) on an orbital shaker overnight at 4°C.