Example sentences for: np

How can you use “np” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PH, pleckstrin homology; PI3K, phosphoinositide 3-Kinase; SH2, Src homology-2, SH3, Src homology-3; XLA, X-linked agammaglobulinemia; xid ; x-linked immunodeficiency; TCR, T cell receptor; sIgM, surface immunoglobulin M; (GAP), GTPase-Activating Protein; GST, Glutathione S-Transferase; SDS, Sodium Dodecyl Sulfate; PBS, phosphate buffered saline; NP40, Nonidet P-40; PAGE, polyacrylamide gel electrophoresis.

  • Control and treated cells were lysed by repeated freeze-thaw cycles with buffer containing 10 mM HEPES (pH 7.5), 1.5 mM Mg(OAc) 2 , 1 mM dithiothreitol (DTT), 0.5% NP40, 5% glycerol, 0.5 mM phenylmethylsulfonyl fluoride, and 10 μg/ml each of the protease inhibitors aprotinin, pepstatin, and leupeptin.

  • Control cDNA clones (GenBank Accession numbers are in brackets) from Arabidopsis thaliana (T76518, T45394, H37673), human cytomegalovirus (NP_039949, NP_039950, NP_039952, NP_039955, NP_039971, NP_039974, NP_039976) E. coli (J04423: BioB, BioC, BioD) and B. subtilis (X04603, M24537, X17013, L38424) were provided by the Scripps Research Institute's microarray facility and were chosen to lack homology to human sequences.

  • Cells were placed on ice, washed once with PBS, then lysed in NP40 Lysis buffer as described above.

  • 1% NP40/20 μM ZnCl 2 /1 mM DTT/250 ng DNA/4.


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