Words similar to np

Example sentences for: np

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Control and treated cells were lysed by repeated freeze-thaw cycles with buffer containing 10 mM HEPES (pH 7.5), 1.5 mM Mg(OAc) 2 , 1 mM dithiothreitol (DTT), 0.5% NP40, 5% glycerol, 0.5 mM phenylmethylsulfonyl fluoride, and 10 μg/ml each of the protease inhibitors aprotinin, pepstatin, and leupeptin.
For GAGase treatment of cell lysates, cells were lysed on ice in NP40 Lysis buffer (1% Nonidet P 40, 150 mM NaCl, 50 mM Tris, pH 8.0) plus protease inhibitors (Complete™ protease inhibitor cocktail) at 2 × 10 6cells/ml.
24 hours following transfection cells were washed once with PBS and lysed in NP40 Lysis Buffer (0.
After denaturation in 6 M and renaturation in 0.187 M guanidine-HCl in HB buffer (25 mM Hepes pH 7.2, 5 mM NaCl, 5 mM MgCl2, 1 mM DTT), the blots were saturated at 4°C in buffer H (20 mM Hepes pH 7.7, 7.75 mM KCl, 0.1 mM EDTA, 25 mM MgCl 2 , 1 mM DTT, 0.05% NP40, 1% dry milk) for 2 hours, and then incubated for 2 hours at 4°C with 50 μl of 35S-methionine-labeled in vitro-translated hCCR4 or luciferase protein (synthesized using a reticulocyte lysate-coupled transcription/translation kit [Promega]).
The GenBank (http://www.ncbi.nlm.nih.gov/Genbank/) accession numbers for the proteins discussed in this paper are ROCK1 (NP_005397) and APP695, 595–596 KM/NL (NP_958817).