Words similar to np
Example sentences for: np
How can you use “np” in a sentence? Here are some example sentences to help you improve your vocabulary:
PH, pleckstrin homology; PI3K, phosphoinositide 3-Kinase; SH2, Src homology-2, SH3, Src homology-3; XLA, X-linked agammaglobulinemia; xid ; x-linked immunodeficiency; TCR, T cell receptor; sIgM, surface immunoglobulin M; (GAP), GTPase-Activating Protein; GST, Glutathione S-Transferase; SDS, Sodium Dodecyl Sulfate; PBS, phosphate buffered saline; NP40, Nonidet P-40; PAGE, polyacrylamide gel electrophoresis.
Control and treated cells were lysed by repeated freeze-thaw cycles with buffer containing 10 mM HEPES (pH 7.5), 1.5 mM Mg(OAc) 2 , 1 mM dithiothreitol (DTT), 0.5% NP40, 5% glycerol, 0.5 mM phenylmethylsulfonyl fluoride, and 10 μg/ml each of the protease inhibitors aprotinin, pepstatin, and leupeptin.
Control cDNA clones (GenBank Accession numbers are in brackets) from Arabidopsis thaliana (T76518, T45394, H37673), human cytomegalovirus (NP_039949, NP_039950, NP_039952, NP_039955, NP_039971, NP_039974, NP_039976) E. coli (J04423: BioB, BioC, BioD) and B. subtilis (X04603, M24537, X17013, L38424) were provided by the Scripps Research Institute's microarray facility and were chosen to lack homology to human sequences.
Cells were placed on ice, washed once with PBS, then lysed in NP40 Lysis buffer as described above.
1% NP40/20 μM ZnCl 2 /1 mM DTT/250 ng DNA/4.
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