Words similar to mhck-a
Example sentences for: mhck-a
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Another feature reported earlier for MHCK-A activation is that myosin II itself stimulates autophosphorylation [ 25 ] . To test whether MHCK-C autophosphorylation is accelerated in the presence of myosin II, the stoichiometry of FLAG-MHCK-C autophosphorylation was evaluated in the presence and absence of myosin II filaments.
Although MHCK-A has been extensively characterized at the biochemical level [ 18 22 25 31 ] , only limited biochemical analysis has been performed with bacterially-expressed subdomains of MHCK-B and MHCK-C [ 17 18 22 ] . The current biochemical results provide strong support for the hypothesis that MHCK-C acts as a MHC kinase in vivo.
With these caveats, the distinct localization patterns for MHCK-A, -B, and -C reported here provide valuable clues as to which spatial and temporal myosin II population may be acted upon by each enzyme.
However, the strong similarity of MHCK-C to MHCK-A and MHCK-B in the conserved catalytic domain and the carboxyl-terminal WD repeat domain suggested that it might represent a third myosin heavy chain kinase in this organism.
The enzymes MHCK-A and MHCK-B have established roles in the control of D. discoideum myosin filament assembly both in vitro and in vivo [ 16 17 24 ] , and Egelhoff, T. T., (unpublished studies).