Words similar to mhck

Example sentences for: mhck

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All fusions were made in the GFP expression vector pTX-GFP [ 36 ] . The construct for MHCK A has been described previously [ 23 ] . Protein sequences for MHCK A, MHCK B, and MHCK C correspond to GenBank entries A55532, AAB50136, and AAC31918, respectively.
The GFP fusions to MHCK A, MHCK B, and MHCK C were constructed by placing GFP at the amino-terminus of each kinase coding region, with GFP fused to codon 2 of each kinase open reading frame.
MHCK domain organization and MHCK C biochemical activity
max of the enzyme 50-fold [ 25 ] . To test for similar autophosphorylation regulation of MHCK-C, we tested the activity of FLAG-MHCK-C with and without an initial autophosphorylation step, towards the peptide substrate MH-1 (a 16-residue peptide corresponding to one of the mapped MHC phosphorylation target sites for MHCK A in the myosin tail).
Co-localization of a MHCK with its apparent substrate does not imply that the kinase, in vivo, is actively phosphorylating its substrate.