Example sentences for: mhck-c

How can you use “mhck-c” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A FLAG epitope was fused to the amino-terminus of MHCK-C at codon 2 using the vector pTX-FLAG [ 36 ] . The resulting plasmid, pTX-MKC2, was transformed into the cell line Ax2 and clonal cell lines were selected with G418 (8 μg/ml) in HL-5 medium.

  • To test this possibility at the biochemical level, we engineered and expressed an epitope-tagged construct that contains an amino-terminal FLAG epitope fused to codon 2 of MHCK-C.

  • These results support the model that autophosphorylation activates MHCK-C.

  • Lysate was adjusted to 300 mM NaCl (to dissociate MHCK-C from binding to particulate material), then subjected to centrifugation at 125,000 × g for 20 min.

  • Dynamic changes in MHC phosphorylation levels within the contractile ring have been reported in dividing sea urchin embryos [ 35 ] , suggesting that MHC phosphorylation as a mechanism regulating furrow myosin II disassembly may occur in other systems besides D. discoideum . We suggest that MHCK-C participates in this regulated myosin II filament disassembly in D. discoideum , and that this function may be regulated at both the cellular and biochemical level.


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