Words similar to mhck-c

Example sentences for: mhck-c

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Western blot analysis with anti-MHCK-C polyclonal antibodies (Figure 2A) and with anti-FLAG monoclonal antibodies (not shown), and Coomassie staining (Figure 2A) confirmed the purification of the expressed protein, migrating at the correct size for MHCK-C.
However, the strong similarity of MHCK-C to MHCK-A and MHCK-B in the conserved catalytic domain and the carboxyl-terminal WD repeat domain suggested that it might represent a third myosin heavy chain kinase in this organism.
To determine whether phosphorylation by MHCK-C affected myosin II assembly properties, samples of purified D. discoideum myosin II were incubated with MHCK-C in the presence and absence of ATP.
max of the enzyme 50-fold [ 25 ] . To test for similar autophosphorylation regulation of MHCK-C, we tested the activity of FLAG-MHCK-C with and without an initial autophosphorylation step, towards the peptide substrate MH-1 (a 16-residue peptide corresponding to one of the mapped MHC phosphorylation target sites for MHCK A in the myosin tail).
Lysate was adjusted to 300 mM NaCl (to dissociate MHCK-C from binding to particulate material), then subjected to centrifugation at 125,000 × g for 20 min.