Words similar to inactivated
Example sentences for: inactivated
How can you use “inactivated” in a sentence? Here are some example sentences to help you improve your vocabulary:
In human cancer, a tumor suppressor gene can be inactivated by mutations, chromosomal loss, promoter silencing, and other means.
All ES cell lines were cultured on mitotically inactivated primary embryonic fibroblasts in ES cell medium supplemented with recombinant leukemia inhibitory factor (LIF) as described elsewhere [ 22 ] . F3 cells were generated by introducing 100 μg of Not1 digested pΔHPRT plasmid into 1.5 × 10 8R1 ES cells by electroporation at 250 volts/500μf/resistance 8 using a BTX ECM600 electroporation system [ 7 ] . Cells containing neo were selected by supplementing the ES cell medium with 250 μg/ml Geneticin (G418, Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).
Placental tissue was digested and trophoblast cells isolated and cultured as described previously [ 15 36 ] . Briefly, the cells collected after digestion were pooled, washed twice in DMEM containing 25 mM HEPES and 4500 mg/L glucose (DMEM-HG), diluted to 10 ml in DMEM-HG supplemented with heat inactivated 5% fetal bovine serum (FBS) and antibiotics (50 μg/ml gentamycin, 100 U/ml penicillin, and 100 μg/ml streptomycin), and viable large nucleated cells (95% in trypan blue exclusion test) were counted.
Collected cells were pooled, washed twice in DMEM-HG, diluted to 10 ml in DMEM-HG supplemented with heat inactivated 5% fetal bovine serum (FBS; Gibco/BRL) and antibiotics (50 μg/ml gentamycin, 100 U/ml penicillin, and 100 μg/ml streptomycin), and viable large nucleated cells (95% in trypan blue exclusion test) were counted.
This glioma is deficient in PTEN and shows constitutive activation of Akt, which can be inactivated by re-introduction of PTEN [ 47 ] .
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