Words similar to inactivated
Example sentences for: inactivated
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All ES cell lines were cultured on mitotically inactivated primary embryonic fibroblasts in ES cell medium supplemented with recombinant leukemia inhibitory factor (LIF) as described elsewhere [ 22 ] . F3 cells were generated by introducing 100 μg of Not1 digested pΔHPRT plasmid into 1.5 × 10 8R1 ES cells by electroporation at 250 volts/500μf/resistance 8 using a BTX ECM600 electroporation system [ 7 ] . Cells containing neo were selected by supplementing the ES cell medium with 250 μg/ml Geneticin (G418, Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).
2A), the Ca 2+current of γ 1 het and γ 1 null cells inactivated more slowly than that of control cells.
All ES cell lines were cultured on mitotically inactivated primary embryonic fibroblasts in ES cell medium supplemented with recombinant leukemia inhibitory factor (LIF) as described elsewhere [ 27].
The other compounds tested were less active, i.e., at 50 μg/mL, they inactivated the virus by less than 50%.
Collected cells were pooled, washed twice in DMEM-HG, diluted to 10 ml in DMEM-HG supplemented with heat inactivated 5% fetal bovine serum (FBS; Gibco/BRL) and antibiotics (50 μg/ml gentamycin, 100 U/ml penicillin, and 100 μg/ml streptomycin), and viable large nucleated cells (95% in trypan blue exclusion test) were counted.