Words similar to inactivate
Example sentences for: inactivate
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The cycling parameters were as follows: (1) 50°C × 30 min, reverse transcription single-strand synthesis (with one, both or neither primer); (2) 95°C × 15 min, activate AmpliTaq polymerase, inactivate RT enzymes; (3) 4°C, add missing primers; (4) 94°C × 30 sec, commence PCR cycling; (5) 56°C × 30 sec; (6) 72°C × 30 sec; (7) go to step 4 (30 cycles in total); (8) 72°C × 10 min.
These bacteria become resistant by a number of mechanisms, such as producing enzymes that hydrolyze or inactivate the antibiotics, producing efflux pumps that transport the antibiotic out of the cell, or modifying their cell wall components so they no longer bind effectively to the antibiotics [ 1 2 3 ] . The most common, least expensive, and effective antibiotics are the β-lactam containing antibiotics, such as the penicillins, cephalosporins, and carbapenems [ 2 4 5 ] . These antibiotics are mechanism-based inhibitors of transpeptidase, a bacterial enzyme required for the production of a strong viable cell wall [ 6 7 ] . In response to the widespread use of β-lactam containing antibiotics, bacteria have acquired the ability to produce β-lactamases, which are enzymes that hydrolyze and inactivate β-lactam containing antibiotics.
ROIs in excess inactivate proteins, disrupt DNA, and oxidize lipids [ 22].
Tyrosine kinases require ATP for their enzymic activity, and thus small molecules that mimic ATP can bind to mutant kinases and inactivate them.
Samples were then incubated at 65°C for 20 min to inactivate kinases, and 25% of the reaction incubated for 30 min with GSK3α or GSK3β in GSK3 kinase buffer (KGB Buffer: 8 mM MOPS, pH7.
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