Example sentences for: heteroduplex

How can you use “heteroduplex” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • (ii) Mutation of the putative RNAseH active site residues in both Hepatitis B Virus (HBV) and duck hepatitis B virus (DHBV) P blocks synthesis of mature hepadnaviral DNA and results in the expected RNA:DNA heteroduplex [ 19, 21, 22, 23].

  • The aim of this study was to determine if the DHBV RNAseH activity was similarly restricted to the endogenous heteroduplex substrate generated during reverse transcription.

  • To exclude the possibility that the DHBV RNAseH simply could not recognize the specific heteroduplex formed by DRF+ RNA and oligonucleotide D2507-, a second pair oligonucleotides (D2453+ and D2543M-Sal) were employed with DRF+ RNA in oligonucleotide-directed RNAseH assays employing permeabilized DHBV core particles.

  • RNAseH cleaves the RNA strand in a RNA:DNA heteroduplex, but it does not cleave DNA or RNA that is not hybridized to DNA.

  • The duplex or heteroduplex substrates were prepared by mixing the labeled top strand with 4 fold excess of the unlabeled bottom strand, heating at 80°C for three minutes, cooling to room temperature and purified by electrophoresis in a 20% non-denaturing polyacrylamide gel with TBE buffer.


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