Words similar to heteroduplex
Example sentences for: heteroduplex
How can you use “heteroduplex” in a sentence? Here are some example sentences to help you improve your vocabulary:
(ii) Mutation of the putative RNAseH active site residues in both Hepatitis B Virus (HBV) and duck hepatitis B virus (DHBV) P blocks synthesis of mature hepadnaviral DNA and results in the expected RNA:DNA heteroduplex [ 19, 21, 22, 23].
The duplex or heteroduplex substrates were prepared by mixing the labeled top strand with 4 fold excess of the unlabeled bottom strand, heating at 80°C for three minutes, cooling to room temperature and purified by electrophoresis in a 20% non-denaturing polyacrylamide gel with TBE buffer.
Analysis of the DNAs on denaturing gels revealed that the negative polarity DNA synthesized by the D715V mutant was annealed to a complementary strand [ 23], indicating that it was in an RNA:DNA heteroduplex.
DHPLC has been developed to screen for DNA variations by separating heteroduplex and homoduplex DNA fragments by ion-pair reverse-phase liquid chromatography [ 22 ] . We used this technique to study eight male patients and one female carrier relative of one of the eight males.
The aim of this study was to determine if the DHBV RNAseH activity was similarly restricted to the endogenous heteroduplex substrate generated during reverse transcription.