Words similar to gfp-nups

Example sentences for: gfp-nups

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The ts phenotype was not, however, accompanied by mislocalization of GFP-Nups after growth for 6 hours at 37°.
Instead, most mutants lost the exclusive, punctate nuclear rim localization, the GFP-Nups were present in areas distinct from the NE and often in discrete foci (Figure 2, panels G and K).
By creating a bank of ts mutants in a doubly tagged GFP-nic96 nup170-GFP S. cerevisiae strain and individually screening the mutants at the non-permissive temperature, we identified 121 ts mutant strains which fail to correctly localize the GFP-Nups at NPCs and/or the NE rim.
(The GFP-Nups did not cause a temperature sensitive phenotype in heterozygous diploids).
Logarithmically growing cultures of npa1-1/sec23 and npa2-1/sec13 were shifted to the non-permissive temperature as before and then fixed and processed for IF with polyclonal antibodies against Kar2p to visualize the ER [ 45 ] . The monoclonal antibody mAb414, which recognizes a subset of Nups distinct from Nic96p and Nup170p [ 46 ] , was also used because the GFP fluorescence of the GFP-Nups was more sensitive to fixation when the GFP-Nups were mislocalized than when they remained at the nuclear rim (unpublished observation).