Words similar to gfp-nups
Example sentences for: gfp-nups
How can you use “gfp-nups” in a sentence? Here are some example sentences to help you improve your vocabulary:
(The GFP-Nups did not cause a temperature sensitive phenotype in heterozygous diploids).
By creating a bank of ts mutants in a doubly tagged GFP-nic96 nup170-GFP S. cerevisiae strain and individually screening the mutants at the non-permissive temperature, we identified 121 ts mutant strains which fail to correctly localize the GFP-Nups at NPCs and/or the NE rim.
We feel this conclusion is also supported by our observation that the distribution of GFP-Nups was altered in mutants for all COPII coat components.
In comparison to our previous genetic screen with a strain expressing only GFP-Nup49p [ 26 ] , these results suggest that increasing the number of GFP-Nups as reporters for NPC integrity effectively increases the likelihood of obtaining a panel of mutants defective in global NPC assembly, stability, and localization.
The ts phenotype was not, however, accompanied by mislocalization of GFP-Nups after growth for 6 hours at 37°.