Words similar to gfp-nups
Example sentences for: gfp-nups
How can you use “gfp-nups” in a sentence? Here are some example sentences to help you improve your vocabulary:
By creating a bank of ts mutants in a doubly tagged GFP-nic96 nup170-GFP S. cerevisiae strain and individually screening the mutants at the non-permissive temperature, we identified 121 ts mutant strains which fail to correctly localize the GFP-Nups at NPCs and/or the NE rim.
Three morphological classes include sec mutants with extended networks of ER membrane, those with accumulated Golgi, and a third with accumulated 80-100 nm secretory vesicles [ 37 ] . The morphological perturbations directly correlate with distinct secretory pathway blocks: ER-Golgi, intra-Golgi, and Golgi-plasma membrane [ 44 ] . A recent study in S. pombe has also shown that sec mutants can affect NE and cell cycle progression [ 55 ] . In addition to sec13 , our screen also identified mutant alleles of SEC23 , SEC27 and BET3 . The mislocalization of GFP-Nups in sec13 and sec23 mutants was coincident with the formation of extensive honeycomb networks of ER.
We feel this conclusion is also supported by our observation that the distribution of GFP-Nups was altered in mutants for all COPII coat components.
In comparison to our previous genetic screen with a strain expressing only GFP-Nup49p [ 26 ] , these results suggest that increasing the number of GFP-Nups as reporters for NPC integrity effectively increases the likelihood of obtaining a panel of mutants defective in global NPC assembly, stability, and localization.
After 4 hours at 34°, the same conditions that cause GFP-Nup mislocalization in the other assayed sec mutants, the GFP-Nups in sec6-4 remained localized to the NPC in the characteristic, punctate NE rim pattern (Figure 7).
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