Words similar to gfp-nups
Example sentences for: gfp-nups
How can you use “gfp-nups” in a sentence? Here are some example sentences to help you improve your vocabulary:
We feel this conclusion is also supported by our observation that the distribution of GFP-Nups was altered in mutants for all COPII coat components.
The ts phenotype was not, however, accompanied by mislocalization of GFP-Nups after growth for 6 hours at 37°.
Three morphological classes include sec mutants with extended networks of ER membrane, those with accumulated Golgi, and a third with accumulated 80-100 nm secretory vesicles [ 37 ] . The morphological perturbations directly correlate with distinct secretory pathway blocks: ER-Golgi, intra-Golgi, and Golgi-plasma membrane [ 44 ] . A recent study in S. pombe has also shown that sec mutants can affect NE and cell cycle progression [ 55 ] . In addition to sec13 , our screen also identified mutant alleles of SEC23 , SEC27 and BET3 . The mislocalization of GFP-Nups in sec13 and sec23 mutants was coincident with the formation of extensive honeycomb networks of ER.
(The GFP-Nups did not cause a temperature sensitive phenotype in heterozygous diploids).
Logarithmically growing cultures of npa1-1/sec23 and npa2-1/sec13 were shifted to the non-permissive temperature as before and then fixed and processed for IF with polyclonal antibodies against Kar2p to visualize the ER [ 45 ] . The monoclonal antibody mAb414, which recognizes a subset of Nups distinct from Nic96p and Nup170p [ 46 ] , was also used because the GFP fluorescence of the GFP-Nups was more sensitive to fixation when the GFP-Nups were mislocalized than when they remained at the nuclear rim (unpublished observation).
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