Words similar to gfp-top

Example sentences for: gfp-top

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However, in cells co-transfected with GFP-TOP1 and m-BTBD2T, the m-BTBD2T staining was nuclear diffuse as well as in nuclear bodies, indicating that m-BTBD2T interacts with GFP-TOP1, which localizes throughout the nucleus (not shown).
To determine if TOP1 co-localizes with BTBD2 in the cytoplasm, GFP-TOP1 lacking an NLS [ 30 ] was co-transfected with full length BTBD2 (m-BTBD2F) or truncated BTBD2 (m-BTBD2T).
Thus, when co-transfected with GFP-TOP1 lacking a NLS, both full length and the N-terminal deleted BTBD2 co-localized with TOP1 to cytoplasmic bodies.
The localization of full length BTBD2 to cytoplasmic bodies was surprising because the cytoplasm is separated from the nucleus where endogenous TOP1 resides and transfected GFP-TOP1 localizes [ 26 27 28 29 30 ] . Because TOP1 and BTBD2 are not predominantly found in the same compartments, they may interact transiently, or they may interact only under specific conditions when they may be in the same compartment.
GFP-TOP1 lacking a NLS (GFP-pTI-6) localizes diffusely within the cytoplasm (Fig.