Words similar to gfp-nup

Example sentences for: gfp-nup

How can you use “gfp-nup” in a sentence? Here are some example sentences to help you improve your vocabulary:

In addition, Western analysis showed no decrease in GFP-Nup protein levels in arrested npa1-1 cells indicating GFP protein stability was not affected (data not shown).
Mutants perturbing GFP-Nup localization could include cells with 1) NPC clusters in NE subregions, 2) mislocalization of the GFP-Nup or aggregated NPC subcomplexes to the cytoplasm or nucleus (due to either a block in new assembly or increased instability of existing NPCs), 3) mislocalization of the GFP-Nup due to indirect perturbation of the ER and NE membrane, or 4) decreased GFP-Nup incorporation followed by turnover resulting in cells with diminished total fluorescence.
However, when the genes encoding GFP-tagged Nups were crossed into these mutant backgrounds, all displayed GFP-Nup mislocalization under non-permissive conditions (Figure 7and data not shown).
After 4 hours at 34°, the same conditions that cause GFP-Nup mislocalization in the other assayed sec mutants, the GFP-Nups in sec6-4 remained localized to the NPC in the characteristic, punctate NE rim pattern (Figure 7).
To screen for GFP-Nup localization, temperature sensitive strains were inoculated individually in 2 ml liquid YPD cultures and grown at 23° for 12-16 hours before shifting to 34° for 4-6 hours.