Words similar to gfp-myosin-ii
Example sentences for: gfp-myosin-ii
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The relative concentrations of GFP-myosin-II were compared for three regions of cells undergoing cytokinesis: the furrow cortex, the polar cortex and the cytoplasm (Figure 1).
To begin to develop a physical model for cytokinesis, we analyzed the changes in the distribution of wild type myosin-II and 3 × Ala GFP-myosin-II during the cell cycle using ratio imaging.
In contrast, the peak concentration of 3 × Ala GFP-myosin-II was 6.2 ± 0.36 (n = 11) relative to the cytoplasm and 7.5 ± 0.49 (n = 11) relative to the cortex at the poles.
The intensities recorded in ratio images are proportional to local GFP-myosin-II concentrations and were used to measure the accumulation and dynamics of myosin-II distribution.
GFP-myosin-II and NLS-GFP were simultaneously expressed from the same extrachromosomal plasmid vector (p102 in the case of wild-type GFP-myosin-II, p122 in the case of 3 × Ala GFP-myosin-II).