Words similar to gfp-myosin-ii

Example sentences for: gfp-myosin-ii

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The relative concentrations of GFP-myosin-II were compared for three regions of cells undergoing cytokinesis: the furrow cortex, the polar cortex and the cytoplasm (Figure 1).
Ratio images of GFP-myosin-II were generated using either the dextran or NLS-GFP, as marker of cytoplasmic volume, and the results compared.
The intensities recorded in ratio images are proportional to local GFP-myosin-II concentrations and were used to measure the accumulation and dynamics of myosin-II distribution.
This number is justified because the thickness of cortical GFP-myosin-II fluorescence is approximately 0.35 μm and is consistent with published electron micrographs of D. discoideum cell cortices [ 35 ] . It should be noted that fluorescence that is defined as cytoplasm also includes fluorescence from the overlying and underlying cortices.
Division of the corrected GFP-myosin-II image by the corrected NLS-GFP image yielded ratio images with pixel intensities that are proportional to local myosin-II concentrations (except in the region occupied by the nucleus).