Words similar to gfp-myosin-ii
Example sentences for: gfp-myosin-ii
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The relative concentrations of GFP-myosin-II were compared for three regions of cells undergoing cytokinesis: the furrow cortex, the polar cortex and the cytoplasm (Figure 1).
GFP-myosin-II and NLS-GFP were simultaneously expressed from the same extrachromosomal plasmid vector (p102 in the case of wild-type GFP-myosin-II, p122 in the case of 3 × Ala GFP-myosin-II).
This number is justified because the thickness of cortical GFP-myosin-II fluorescence is approximately 0.35 μm and is consistent with published electron micrographs of D. discoideum cell cortices [ 35 ] . It should be noted that fluorescence that is defined as cytoplasm also includes fluorescence from the overlying and underlying cortices.
Ratio images of GFP-myosin-II were generated using either the dextran or NLS-GFP, as marker of cytoplasmic volume, and the results compared.
The peak concentration of wild type GFP-myosin-II was 1.8 ± 0.14 relative to the polar cortex and 2.0 ± 0.14 relative to the cytoplasm (n = 12).