Words similar to gfp-n
Example sentences for: gfp-n
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However, treatment with LMB completely shifts GFP-N200 from the cytoplasm to the nucleus, implying GFP-N200 may shuttle between the nucleus and the cytoplasm, consistent with the existence of both the NES and the NLS in GFP-N200 (Fig 6and Fig 7).
Unlike wild-type GFP-N346, GFP-N346 NLSmut1(R113S/K114N) and GFP-N346 NLSmut3(K205T/R206S/K208T) are totally localized to the cytoplasm, still forming speckles (Fig 7B).
Further, we do not know whether the cytoplasmic speckles formed by GFP-N346 NLSmut1and GFP-N346 NLSmut3are in any way related to the nuclear speckles of wild-type GFP-N346.
The different domain compositions of GFP-346, GFP-N280 and GFP-N200 may explain the distinct localization patterns of these mutant proteins.
Deletion constructs, GFP-N346, GFP-N280 and GFP-N200, all contain both the NLS and the NES, but their subcellular localization patterns are strikingly different.