Words similar to gfp-n

Example sentences for: gfp-n

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To determine whether the RING finger plays a role in mediating nuclear import, we mutated two key zinc-binding cysteine residues at the RING finger domain of GFP-N346 (C158A/C161A, N346 RINGmut) to destroy the RING finger structure (Fig 7A).
The different domain compositions of GFP-346, GFP-N280 and GFP-N200 may explain the distinct localization patterns of these mutant proteins.
Furthermore, we also chose to mutate a forth positively charged amino acid cluster at amino acid 358 to 360, which corresponds to the Arabidopsis COP1 NLS [ 17 ] . Since GFP-N346 exhibited clear and consistent nuclear localized pattern with speckles most resembling the full-length MmCOP1 nuclear localization pattern, it was used as the starting point for the site-directed mutagenesis studies (Fig 7A).
However, the distance between site 1 and site 3 in GFP-N346 is more than 90 amino acids apart, separated by the RING finger domain (Fig 7A).
GFP-N280 (amino acid 71-350), which contains the RING finger and the coiled-coil domain, but not the sequence corresponding to the NLS in AtCOP1, is also solely localized in the nucleus (Fig 5Aand 5B).