Words similar to gfp-nic

Example sentences for: gfp-nic

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In contrast, both mutants showed a temperature dependent mislocalization of GFP-Nic96p and Nup170p-GFP over a four hour time course (Figure 3).
To determine if the overlap between npa mutants and the secretory pathway was restricted to gene products affecting the ER, the sec6-4 mutant perturbing Golgi to plasma membrane transport [ 52 ] was crossed into the GFP-nic96 nup170-GFP strain.
Cells for photography were grown in media lacking histidine, as the HIS3 linked GFP-tag of GFP-nic96 showed some instability in the nup170-GFP background.
Wild type GFP-nic96 nup170-GFP haploid strains were mutagenized to generate a bank of ts mutant strains (Figure 1A; see Materials and Methods).
A fraction of the total cellular Sec13p is isolated in a NPC subcomplex containing Nup84p, Nup85p, Nup120p, Nup145p-C, and Seh1p (a Sec13-related protein) [ 40 41 ] . Hurt and coworkers have also reported that ts sec13 mutants show enhanced growth defects when combined with a nup85Δ mutant, and result in the mislocalization of GFP-Nup49p to NE clusters and cytoplasmic foci [ 41 ] . This correlates with our observations of GFP-Nic96p and Nup170p-GFP in our sec13/npa2 mutant.