Words similar to gfp-nic

Example sentences for: gfp-nic

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Wild type GFP-nic96 nup170-GFP haploid strains were mutagenized to generate a bank of ts mutant strains (Figure 1A; see Materials and Methods).
A fraction of the total cellular Sec13p is isolated in a NPC subcomplex containing Nup84p, Nup85p, Nup120p, Nup145p-C, and Seh1p (a Sec13-related protein) [ 40 41 ] . Hurt and coworkers have also reported that ts sec13 mutants show enhanced growth defects when combined with a nup85Δ mutant, and result in the mislocalization of GFP-Nup49p to NE clusters and cytoplasmic foci [ 41 ] . This correlates with our observations of GFP-Nic96p and Nup170p-GFP in our sec13/npa2 mutant.
In contrast, both mutants showed a temperature dependent mislocalization of GFP-Nic96p and Nup170p-GFP over a four hour time course (Figure 3).
Like GFP-Nic96p and Nup170p-GFP, the localization of Nup116p and Pom152p changed from a punctate, nuclear rim staining to cytoplasmic foci when shifted to the non-permissive temperature (data not shown).
By creating a bank of ts mutants in a doubly tagged GFP-nic96 nup170-GFP S. cerevisiae strain and individually screening the mutants at the non-permissive temperature, we identified 121 ts mutant strains which fail to correctly localize the GFP-Nups at NPCs and/or the NE rim.