Words similar to gfp-nic

Example sentences for: gfp-nic

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By creating a bank of ts mutants in a doubly tagged GFP-nic96 nup170-GFP S. cerevisiae strain and individually screening the mutants at the non-permissive temperature, we identified 121 ts mutant strains which fail to correctly localize the GFP-Nups at NPCs and/or the NE rim.
Like GFP-Nic96p and Nup170p-GFP, the localization of Nup116p and Pom152p changed from a punctate, nuclear rim staining to cytoplasmic foci when shifted to the non-permissive temperature (data not shown).
In addition to the mislocalization of GFP-Nic96p and Nup170p-GFP, Nup116p, Pom152p and Nups recognized by mAb414 were also perturbed in the mutants characterized to date.
Crossing a nup170-GFP:URA3 haploid isolate to SWY1695 generated doubly tagged GFP-nic96 nup170-GFP parental strains.
Wild type GFP-nic96 nup170-GFP haploid strains were mutagenized to generate a bank of ts mutant strains (Figure 1A; see Materials and Methods).