Words similar to gfp-nic

Example sentences for: gfp-nic

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Wild type GFP-nic96 nup170-GFP haploid strains were mutagenized to generate a bank of ts mutant strains (Figure 1A; see Materials and Methods).
In the doubly tagged GFP-nic96 nup170-GFP strains, both GFP-Nic96p and Nup170p-GFP were targeted to the NE and showed punctate rim-staining characteristic of NPC localization (Figure 2).
To determine if the overlap between npa mutants and the secretory pathway was restricted to gene products affecting the ER, the sec6-4 mutant perturbing Golgi to plasma membrane transport [ 52 ] was crossed into the GFP-nic96 nup170-GFP strain.
A fraction of the total cellular Sec13p is isolated in a NPC subcomplex containing Nup84p, Nup85p, Nup120p, Nup145p-C, and Seh1p (a Sec13-related protein) [ 40 41 ] . Hurt and coworkers have also reported that ts sec13 mutants show enhanced growth defects when combined with a nup85Δ mutant, and result in the mislocalization of GFP-Nup49p to NE clusters and cytoplasmic foci [ 41 ] . This correlates with our observations of GFP-Nic96p and Nup170p-GFP in our sec13/npa2 mutant.
Like GFP-Nic96p and Nup170p-GFP, the localization of Nup116p and Pom152p changed from a punctate, nuclear rim staining to cytoplasmic foci when shifted to the non-permissive temperature (data not shown).