Words similar to dsrna
Example sentences for: dsrna
How can you use “dsrna” in a sentence? Here are some example sentences to help you improve your vocabulary:
As the proposed mechanisms by which the formation of long duplex dsRNA can potentially affect gene regulation are so varied [ 4, 5, 6, 7, 8, 9, 10, 11, 12], it is difficult to draw inferences regarding function without further experiments.
The anti-F dsRNA also had no effect on P protein levels, suggesting that such dsRNAs do not activate a general antiviral response that might abrogate all viral mRNA translation.
First, to test the effectiveness of the anti-F dsRNA intracellularly, we probed the infected cell monolayer with anti-F antibody by indirect immunofluorescence (Fig.
The negative control luciferase RNA was the same as the double-stranded GL3 RNA described previously [ 17 ] . The RNAs were annealed in vitro to form double-stranded RNA (dsRNA) [ 18 ] and electroporation was carried out essentially as described [ 32 ] . In brief, 3 μg dsRNA in 800 μl of incomplete cytomix [ 32 ] was added to infected RBC (at 10-15% parasitemia), and electroporation was performed using a Bio-Rad Gene Pulsar unit at settings of 200 Ω, 2 kV, and 25 μF.
Over the last decade, RNA interference (RNAi), mediated by short interfering double-stranded RNA molecules (siRNA or dsRNA), has been gradually recognized as a major mechanism of post-transcriptional gene silencing (PTGS) in species as diverse as plants, Drosophila, and C. elegans [ 1 ] . Recently, 21-nucleotide long dsRNA molecules corresponding to specific mRNA sequences, when introduced into mammalian cells in culture, have been shown to be highly effective in degrading the cognate mRNAs and thus abrogating the expression of the corresponding proteins [ 2 ] . Although the exact mechanism of PTGS is currently unknown and is an area of intense research, the successful use of this phenomenon in cultured mammalian cells has raised the exciting prospect that it can be used as a simple strategy for phenotypic ablation of mammalian gene function ex vivo without the time-consuming and expensive construction of transgenic animals.
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