Words similar to dsdna
Example sentences for: dsdna
How can you use “dsdna” in a sentence? Here are some example sentences to help you improve your vocabulary:
Thus, with regard to primary protein sequence (Figure 1), inferred subunit structure (Figure 2and data not shown), strict requirement for meiotic recombination (Figure 2, Table 1), and dependence upon its active site tyrosine (Table 1), Rec12 protein is a structurally and functionally conserved member of the Spo11 family of enzymes that catalyze meiotic dsDNA cleavage to initiate recombination [ 11 ] .
Meiotically induced, Spo11 (Rec12)-dependent double-strand DNA (dsDNA) breaks have been demonstrated in budding yeast and fission yeast [ 12 13 14 15 ] . Spo11 almost certainly catalyzes these breaks, as it shares high homology with the A subunit of Topoisomerase VI of Sulfolobus shibatae [ 16 17 ] and it becomes linked by a phosphotyrosine linkage to the 5' end of the cleaved DNA [ 18 19 20 21 ] . Spo11 homologs have been identified in a wide range of eukaryotes, and available evidence suggests that initiation of meiotic recombination via Spo11-dependent dsDNA break formation is conserved [ 22 23 24 25 ] . Our results with the fission yeast S. pombe fully support this view (see results and discussion).
The ERF protein of phage P22 is involved in the circularization of the linear dsDNA phage genome upon entry into the host cell [ 21 22 23 ] . Experimental studies have shown that, mutations in ERF are complemented by Redβ and that in vitro ERF adopts quaternary structures analogous to those of Redβ and RecT [ 14 17 24 25 ] . However, in the comprehensive analysis of the RecT/Redβ superfamily no statistically significant similarity could be detected between these proteins and the ERF proteins.
The simplest interpretation is that Rec12 functions in a multisubunit protein complex; a complex in which other components are required either for efficient catalysis of dsDNA breaks or for correct subcellular localization of Rec12.
The role of Spo11 (Rec12) in the initiation of meiotic recombination via catalysis of dsDNA breaks is conserved and likely universal among eukaryotes.
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