Words similar to dsdna
Example sentences for: dsdna
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For each dsDNA species present in the starting material, these reactions generate two dsDNAs with T7 promoters at opposite ends.
Experimental studies have shown that the strand transfer reaction mediated by RecT and its binding to dsDNA are sensitive to Mg +2concentrations and it was proposed that the levels of free Mg +2could regulate RecT activity [ 4 ] . Similarly, Redβ has been shown to promote single strand annealing in a Mg +2-dependent manner [ 20 ] . In this context, the conservation of the two C-terminal acidic residues in the majority of members of this superfamily suggests that these might be involved in the coordination of Mg +2and implies that the metal ion-dependent conformational switching is likely to be a generic feature of this family.
Spo11 protein has potential functions in addition to catalyzing dsDNA breaks.
The recE gene product of the Rac prophage converts dsDNA ends into 3' protruding single-stranded form and the recT gene product promotes recombination by annealing them with a homologous DNA in its vicinity (for review, see [ 7 8 ] ). This recombination may result in one progeny DNA (non-conservative recombination) or two progeny DNAs (conservative double-strand break repair) [ 9 ] . In a recBC sbcBC background, a ds end stimulates homologous recombination that results in only one progeny DNA (non-conservative recombination) [ 10 ] . Analysis of the stimulation of recombination by replication (for review, see [ 11 ] ) and analysis of altered chromosomal replication (for review, see [ 12 ] ) led to the proposal that a chromosomal ds break formed during replication fork arrest triggers homologous recombination, which would reconstitute a replication fork (for review, see [ 5 ] ).
The simplest interpretation is that Rec12 functions in a multisubunit protein complex; a complex in which other components are required either for efficient catalysis of dsDNA breaks or for correct subcellular localization of Rec12.
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