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Example sentences for: dsrnai

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We have attempted to alter the concentrations of components by transient transfection with plasmids encoding wild type and mutant proteins and/or with dsRNAi that can block production of proteins from endogenous or exogenous genes.
Experiments with Arr dsRNAi clearly establish a requirement for endogenous Arr to generate the synergistic response to exogenous Wg plus DFz2 in S2 cells (Figure 2).
To explore these issues, we used dsRNAi to reduce the levels of Arr and DFz2 RNAs in S2 cells in which Wg signaling was stimulated, since others have shown that the expression of endogenous genes in these cells can be eliminated or reduced by direct application of T7-transcribed dsRNAs [ 15 16 ] . Using this approach (Figure 2), we found that the previously observed, approximately 30-fold stimulation of luciferase activity by the Wg expression plasmid was reduced by 70% or more when S2 cells were co-transfected with inhibitory RNAs for Arr, DFz2, or both (Figure 2A), implying that endogenous levels of both types of receptor are required for an efficient response to Wg.
The use of dsRNAi helps to refine the nature of such interactions and their effects on downstream events in the signaling circuitry.
Moreover, we found that S2 cells treated with dsRNAi against both Arr and Dsh further impaired the Wg and DFz2 signaling activity up to 95% (Figure 2B).