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Example sentences for: dsrnai

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The use of dsRNAi helps to refine the nature of such interactions and their effects on downstream events in the signaling circuitry.
To ask whether LRP6 can replace the requirement for endogenous Arr in the synergy between Wg and DFz2, as observed earlier in Figure 2B, we again used Arr-specific dsRNAi to try to block the strong activation of signaling by Wg plus DFz2, in the presence and absence of the LRP6 constructs (Figure 5C).
8 to 16 hours after dsRNAi treatment, cells were transfected with different constructs and reporters.
To explore these issues, we used dsRNAi to reduce the levels of Arr and DFz2 RNAs in S2 cells in which Wg signaling was stimulated, since others have shown that the expression of endogenous genes in these cells can be eliminated or reduced by direct application of T7-transcribed dsRNAs [ 15 16 ] . Using this approach (Figure 2), we found that the previously observed, approximately 30-fold stimulation of luciferase activity by the Wg expression plasmid was reduced by 70% or more when S2 cells were co-transfected with inhibitory RNAs for Arr, DFz2, or both (Figure 2A), implying that endogenous levels of both types of receptor are required for an efficient response to Wg.
Signaling via LRP6ΔN, however, while dependent on Arm as expected, is not dependent on Dsh or DFz2, as judged by the use of dsRNAi (Figure 5A).